@inbook{cb3690cc7bb7448b928a41b0cb4be867,
title = "Genome editing in mice using TALE nucleases",
abstract = "Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently.",
keywords = "Gene targeting, Knock-in, Knockout, Mouse, One-cell embryo, TALEN",
author = "Benedikt Wefers and Christina Brandl and Oskar Ortiz and Wolfgang Wurst and Ralf K{\"u}hn",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-2932-0_1",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "229--243",
booktitle = "Methods in Molecular Biology",
}