Genistein affects the expression of genes involved in blood pressure regulation and angiogenesis in primary human endothelial cells

R. Ambra, G. Rimbach, S. De Pascual Teresa, D. Fuchs, U. Wenzel, H. Daniel, F. Virgili

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Background: Several lines of evidence suggest that the dietary isoflavone genistein (Gen) has beneficial effects with regard to cardiovascular disease and in particular on aspects related to blood pressure and angiogenesis. The biological action of Gen may be, at least in part, attributed to its ability to affect cell signalling and response. However, so far, most of the molecular mechanisms underlying the activity of Gen in the endothelium are unknown. Methods and results: To examine the transcriptional response to 2.5 μM Gen on primary human endothelial cells (HUVEC), we applied cDNA array technology both under baseline condition and after treatment with the pro-atherogenic stimulus, copper-oxidized LDL. The alteration of the expression patterns of individual transcripts was substantiated using either RT-PCR or Northern blotting. Gen significantly affected the expression of genes encoding for proteins centrally involved in the vascular tone such as endothelin-converting enzyme-1, endothelin-2, estrogen related receptor α and atrial natriuretic peptide receptor A precursor. Furthermore, Gen countered the effect of oxLDL on mRNA levels encoding for vascular endothelial growth factor receptor 165, types 1 and 2. Conclusions: Our data indicate that physiologically achievable levels of Gen change the expression of mRNA encoding for proteins involved in the control of blood pressure under baseline conditions and reduce the angiogenic response to oxLDL in the endothelium.

Original languageEnglish
Pages (from-to)35-43
Number of pages9
JournalNutrition, Metabolism and Cardiovascular Diseases
Volume16
Issue number1
DOIs
StatePublished - Jan 2006

Keywords

  • Angiogenesis
  • Blood pressure
  • Endothelial cells
  • Gene expression
  • Genistein

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