Genetically engineered V79 chinese hamster cell expression of purified cytochrome P‐450iib1 monooxygenase activity

K. L. Platt, E. Molitor, J. Döhmer, S. Dogra, F. Oesch

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55 Scopus citations

Abstract

Chinese hamster V79 fibroblasts, frequently used as target cells in short‐term tests for mutagenicity, do not possess measurable monooxygenase activity; in particular, enzymatic oxidation of testosterone (T) cannot be demonstrated. If these V79 cells, however, had been transfected with the cDNA‐encoding rat liver cytochrome P‐450IIB1 under control of the SV40 early promoter, they stably expressed monooxygenase activity. These so‐called SD1 cells then oxidatively metabolized T at a rate of 27 pmol/mg protein/min, converting it to 16α‐ and 16β‐hydroxy‐T as well as 4‐androsten‐3,17‐dione as sole metabolites in a ratio of 1.1:1.0:1.6. The regio‐ and stereoselective conversion of T by SD1 cells, as well as the quantitative distribution of the metabolites, corresponds well with the results reported for pure cytochrome P‐450IIB1 in a reconstituted system.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Biochemical Toxicology
Volume4
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Keywords

  • Chinese Hamster Fibroblasts
  • Expression of Cytochrome P‐450IIB1
  • Gene Transfer
  • Testosterone Metabolism

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