Genetically encoded FRET probe for PKC activity based on pleckstrin

Andreas Schleifenbaum, Gunter Stier, Alexander Gasch, Michael Sattler, Garsten Schultz

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


We developed a probe for investigating protein kinase C (PKC) activity in living cells. The probe is based on a fragment of pleckstrin enclosed by two FRET-capable fluorophores. PKC activity modulation was reliably followed by FRET change in vitro and in vivo. The probe responds quickly to PKC activation by phorbol ester. FRET changes were reversible when PKC inhibitors were administered. Stimulation of cellular signaling pathways using histamine or bradykinin triggered PKC in a physiologically relevant way.

Original languageEnglish
Pages (from-to)11786-11787
Number of pages2
JournalJournal of the American Chemical Society
Issue number38
StatePublished - 29 Sep 2004
Externally publishedYes


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