Genetic transformation of intact cells of Lactobacillus curvatus Lc2‐c and Lact. sake Ls2 by elctroporation

W. Gaier, R. F. Vogel, W. P. Hammes

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

An electroporation‐induced transformation system was developed for two raw sausage starter organisms: Lactobacillus curvatus Lc2‐c and Lact. sake Ls2. Several plasmids of different origin (pAMβ1, pIL253, pNZ12 and pLP825) were tested for transformation and expression of their resistance determinants. Transfer rates were strongly dependent on growth phase and voltage. Several parameters were studied with possible influence on trasformation efficiencies.

Original languageEnglish
Pages (from-to)81-83
Number of pages3
JournalLetters in Applied Microbiology
Volume11
Issue number2
DOIs
StatePublished - Aug 1990
Externally publishedYes

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