TY - JOUR
T1 - Genetic profile of 22 pancreatic carcinoma cell lines
T2 - Analysis of K-ras, p53, p16 and DPC4/Smad4
AU - Moore, Patrick S.
AU - Sipos, Bence
AU - Orlandini, Simonetta
AU - Sorio, Claudio
AU - Real, Francisco X.
AU - Lemoine, Nicholas R.
AU - Gress, Thomas
AU - Bassi, Claudio
AU - Klöppel, Günter
AU - Kalthoff, Holger
AU - Ungefroren, Hendrik
AU - Löhr, Matthias
AU - Scarpa, Aldo
PY - 2001
Y1 - 2001
N2 - The K-ras, p53, p16 and DPC4 genes are among those most frequently altered in pancreatic ductal carcinoma. We analyzed 22 cell lines for alterations in these genes by direct sequence analysis and methylation-specific polymerase chain reaction. These cell lines showed mutations in K-ras and p53 at frequencies of 91% and 95%, respectively. Alterations in p16INK4a were found in all cases and included nine homozygous deletions, seven mutations and promoter methylation in six cases. Eight cell lines (36%) had an alteration of DPC4, including one mutation and seven homozygous deletions. The most typical mutational profile involved K-ras, p53, and p16INK4a, concurrently aberrated in 20 cases (91%). Eight cell lines had alterations in all four genes. Inactivation of DPC4 was always accompanied by alteration of all of the other three genes. This comprehensive data regarding the cumulative genetic alterations in pancreatic carcinoma cell lines will be of great value for studies involving drug sensitivity or resistance that may be associated with inactivation of a particular gene or molecular pathway.
AB - The K-ras, p53, p16 and DPC4 genes are among those most frequently altered in pancreatic ductal carcinoma. We analyzed 22 cell lines for alterations in these genes by direct sequence analysis and methylation-specific polymerase chain reaction. These cell lines showed mutations in K-ras and p53 at frequencies of 91% and 95%, respectively. Alterations in p16INK4a were found in all cases and included nine homozygous deletions, seven mutations and promoter methylation in six cases. Eight cell lines (36%) had an alteration of DPC4, including one mutation and seven homozygous deletions. The most typical mutational profile involved K-ras, p53, and p16INK4a, concurrently aberrated in 20 cases (91%). Eight cell lines had alterations in all four genes. Inactivation of DPC4 was always accompanied by alteration of all of the other three genes. This comprehensive data regarding the cumulative genetic alterations in pancreatic carcinoma cell lines will be of great value for studies involving drug sensitivity or resistance that may be associated with inactivation of a particular gene or molecular pathway.
KW - Carcinoma cell lines
KW - DPC4/Smad4
KW - K-ras
KW - P16
KW - P53
KW - Pancreas
UR - http://www.scopus.com/inward/record.url?scp=0035207991&partnerID=8YFLogxK
U2 - 10.1007/s004280100474
DO - 10.1007/s004280100474
M3 - Article
C2 - 11787853
AN - SCOPUS:0035207991
SN - 0945-6317
VL - 439
SP - 798
EP - 802
JO - Virchows Archiv
JF - Virchows Archiv
IS - 6
ER -