Genetic polymorphisms at Gli-Dt gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillary electrophoresis

Y. M. Yan, S. L.K. Hsam, J. Z. Yu, Y. Jiang, F. J. Zeller

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Gliadin variation at Gli-Dt1 and Gli-Dt2 loci in 198 Aegilops tauschii accessions was studied by acid polyacrylamide gel electrophoresis (A-PAGE) and capillary electrophoresis (CE). High genetic polymorphisms were found at both gliadin coding loci, revealing a total of 184 and 169 gliadin variants at the Gli-Dt1 and Gli-Dt2 loci, respectively. In particular, 12 gliadin blocks encoded by different alleles were apparently expressed and readily identified in six synthetic hexaploids produced by hybridization between Triticum durum and Ae. tauschii accessions. Compared with Ae. tauschii ssp. eusquarrosa, the gliadin profile of the D genome in Ae. tauschii ssp. strangulata more resembles that of T. aestivum, supporting the view that the subspecies strangulata is the most likely progenitor of bread wheat. Capillary electrophoresis analysis showed that the method is capable of separating and characterizing gliadins with speed, in high resolution using small sample amounts, and is well-suited to detect protein alleles and to identify desirable genotypes in wheat quality improvement.

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalPlant Breeding
Volume122
Issue number2
DOIs
StatePublished - Apr 2003

Keywords

  • A-PAGE
  • Aegilops tauschii
  • Bread quality improvement
  • Capillary electrophoresis
  • Gliadins
  • Triticum aestivum

Fingerprint

Dive into the research topics of 'Genetic polymorphisms at Gli-Dt gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillary electrophoresis'. Together they form a unique fingerprint.

Cite this