Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci

Xueli An; Qiaoyun Li; Yueming Yan; Yinghua Xiao; S. L.K. Hsam; F. J. Zeller

doi:10.1007/s10681-005-9002-6

Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci

Xueli An, Qiaoyun Li, Yueming Yan, Yinghua Xiao, S. L.K. Hsam, F. J. Zeller

Chair of Plant Breeding

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Abstract

High and low molecular weight glutenin subunit (HMW-GS and LMW-GS) compositions of 270 European spelts, 15 Iranian spelts and 25 bread wheat cultivars were analyzed by one- and two-dimensional gel electrophoresis. The results revealed a total of 22 HMW-GS alleles (4 at Glu-A1, 11 at Glu-B1 and 7 at Glu-D1) and 32 allele combinations among the three Glu-1 loci. Two major genotypes of HMW-GS: 1, 13+16, 2+12 and 1, 6.1+22.1, 2+12 were found to occur in Central European spelt wheat cultivars and landraces at higher frequencies of 35 and 28%, respectively. The Glu-B1 locus displayed the greatest variation and genetic diversity index (H) was 0.69 whereas Glu-A1 and Glu-D1 showed H index values of 0.26 and 0.19, respectively. The dendrogram constructed by HMW and LMW glutenin subunit bands revealed that European spelts form a separated cluster from common wheat suggesting that spelt and common wheat form distinct groups. In addition, all 15 Iranian spelt land variety accessions differed from European spelts and possessed similar HMW-GS alleles to common wheat. Because of a wider polymorphism Central European spelt wheats are an important genetic reserviour for improving common wheat quality.

Original language	English
Pages (from-to)	193-201
Number of pages	9
Journal	Euphytica
Volume	146
Issue number	3
DOIs	https://doi.org/10.1007/s10681-005-9002-6
State	Published - Dec 2005

Keywords

2-D electrophoresis
Cluster analysis
HMW-GS
LMW-GS
SDS-PAGE
Triticum spelta L.

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10.1007/s10681-005-9002-6

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@article{d39d588704d149a78c9e3d162e520533,

title = "Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci",

abstract = "High and low molecular weight glutenin subunit (HMW-GS and LMW-GS) compositions of 270 European spelts, 15 Iranian spelts and 25 bread wheat cultivars were analyzed by one- and two-dimensional gel electrophoresis. The results revealed a total of 22 HMW-GS alleles (4 at Glu-A1, 11 at Glu-B1 and 7 at Glu-D1) and 32 allele combinations among the three Glu-1 loci. Two major genotypes of HMW-GS: 1, 13+16, 2+12 and 1, 6.1+22.1, 2+12 were found to occur in Central European spelt wheat cultivars and landraces at higher frequencies of 35 and 28%, respectively. The Glu-B1 locus displayed the greatest variation and genetic diversity index (H) was 0.69 whereas Glu-A1 and Glu-D1 showed H index values of 0.26 and 0.19, respectively. The dendrogram constructed by HMW and LMW glutenin subunit bands revealed that European spelts form a separated cluster from common wheat suggesting that spelt and common wheat form distinct groups. In addition, all 15 Iranian spelt land variety accessions differed from European spelts and possessed similar HMW-GS alleles to common wheat. Because of a wider polymorphism Central European spelt wheats are an important genetic reserviour for improving common wheat quality.",

keywords = "2-D electrophoresis, Cluster analysis, HMW-GS, LMW-GS, SDS-PAGE, Triticum spelta L.",

author = "Xueli An and Qiaoyun Li and Yueming Yan and Yinghua Xiao and Hsam, {S. L.K.} and Zeller, {F. J.}",

note = "Funding Information: This research was financially supported by research grants (No. JY-03-A-13 and No. 5002003) from National Special Project on Transgenic Plants and Industrialization Research of China and Beijing Natural Science Foundation, respectively.",

year = "2005",

month = dec,

doi = "10.1007/s10681-005-9002-6",

language = "English",

volume = "146",

pages = "193--201",

journal = "Euphytica",

issn = "0014-2336",

publisher = "Springer Science and Business Media B.V.",

number = "3",

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TY - JOUR

T1 - Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci

AU - An, Xueli

AU - Li, Qiaoyun

AU - Yan, Yueming

AU - Xiao, Yinghua

AU - Hsam, S. L.K.

AU - Zeller, F. J.

N1 - Funding Information: This research was financially supported by research grants (No. JY-03-A-13 and No. 5002003) from National Special Project on Transgenic Plants and Industrialization Research of China and Beijing Natural Science Foundation, respectively.

PY - 2005/12

Y1 - 2005/12

N2 - High and low molecular weight glutenin subunit (HMW-GS and LMW-GS) compositions of 270 European spelts, 15 Iranian spelts and 25 bread wheat cultivars were analyzed by one- and two-dimensional gel electrophoresis. The results revealed a total of 22 HMW-GS alleles (4 at Glu-A1, 11 at Glu-B1 and 7 at Glu-D1) and 32 allele combinations among the three Glu-1 loci. Two major genotypes of HMW-GS: 1, 13+16, 2+12 and 1, 6.1+22.1, 2+12 were found to occur in Central European spelt wheat cultivars and landraces at higher frequencies of 35 and 28%, respectively. The Glu-B1 locus displayed the greatest variation and genetic diversity index (H) was 0.69 whereas Glu-A1 and Glu-D1 showed H index values of 0.26 and 0.19, respectively. The dendrogram constructed by HMW and LMW glutenin subunit bands revealed that European spelts form a separated cluster from common wheat suggesting that spelt and common wheat form distinct groups. In addition, all 15 Iranian spelt land variety accessions differed from European spelts and possessed similar HMW-GS alleles to common wheat. Because of a wider polymorphism Central European spelt wheats are an important genetic reserviour for improving common wheat quality.

AB - High and low molecular weight glutenin subunit (HMW-GS and LMW-GS) compositions of 270 European spelts, 15 Iranian spelts and 25 bread wheat cultivars were analyzed by one- and two-dimensional gel electrophoresis. The results revealed a total of 22 HMW-GS alleles (4 at Glu-A1, 11 at Glu-B1 and 7 at Glu-D1) and 32 allele combinations among the three Glu-1 loci. Two major genotypes of HMW-GS: 1, 13+16, 2+12 and 1, 6.1+22.1, 2+12 were found to occur in Central European spelt wheat cultivars and landraces at higher frequencies of 35 and 28%, respectively. The Glu-B1 locus displayed the greatest variation and genetic diversity index (H) was 0.69 whereas Glu-A1 and Glu-D1 showed H index values of 0.26 and 0.19, respectively. The dendrogram constructed by HMW and LMW glutenin subunit bands revealed that European spelts form a separated cluster from common wheat suggesting that spelt and common wheat form distinct groups. In addition, all 15 Iranian spelt land variety accessions differed from European spelts and possessed similar HMW-GS alleles to common wheat. Because of a wider polymorphism Central European spelt wheats are an important genetic reserviour for improving common wheat quality.

KW - 2-D electrophoresis

KW - Cluster analysis

KW - HMW-GS

KW - LMW-GS

KW - SDS-PAGE

KW - Triticum spelta L.

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U2 - 10.1007/s10681-005-9002-6

DO - 10.1007/s10681-005-9002-6

M3 - Article

AN - SCOPUS:33644784000

SN - 0014-2336

VL - 146

SP - 193

EP - 201

JO - Euphytica

JF - Euphytica

IS - 3

ER -

Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.) revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci

Abstract

Keywords

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