Generation of targeted mouse mutants by embryo microinjection of TALENs

Benedikt Wefers; Oskar Ortiz; Wolfgang Wurst; Ralf Kühn

doi:10.1016/j.ymeth.2014.01.002

Generation of targeted mouse mutants by embryo microinjection of TALENs

Benedikt Wefers, Oskar Ortiz, Wolfgang Wurst, Ralf Kühn

Chair of Developmental Genetics

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.

Original language	English
Pages (from-to)	94-101
Number of pages	8
Journal	Methods
Volume	69
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2014.01.002
State	Published - 2014

Keywords

Gene targeting
Knockin
Knockout
Mouse
One-cell embryo
TALEN

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10.1016/j.ymeth.2014.01.002

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title = "Generation of targeted mouse mutants by embryo microinjection of TALENs",

abstract = "Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.",

keywords = "Gene targeting, Knockin, Knockout, Mouse, One-cell embryo, TALEN",

author = "Benedikt Wefers and Oskar Ortiz and Wolfgang Wurst and Ralf K{\"u}hn",

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AU - Wefers, Benedikt

AU - Ortiz, Oskar

AU - Wurst, Wolfgang

AU - Kühn, Ralf

PY - 2014

Y1 - 2014

N2 - Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.

AB - Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.

KW - Gene targeting

KW - Knockin

KW - Knockout

KW - Mouse

KW - One-cell embryo

KW - TALEN

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VL - 69

SP - 94

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JO - Methods

JF - Methods

IS - 1

ER -

Generation of targeted mouse mutants by embryo microinjection of TALENs

Abstract

Keywords

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