Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines

Fangfang Zhang, Anna B. Meier, Peter Lipp, Karl Ludwig Laugwitz, Tatjana Dorn, Alessandra Moretti

Research output: Contribution to journalArticlepeer-review

Abstract

TRPM4 is a Ca2+-activated channel mediating the transport of monovalent cations across the cell membrane. Mutations in the TRPM4 gene have been associated with cardiac arrhythmias in humans. Using CRISPR/Cas9 gene editing technology, we established two TRPM4 knockout human iPSC lines – one heterozygous (MRli003-A-3) and one homozygous (MRli003-A-4) – by inserting a frameshift mutation in exon 2 of the TRPM4 gene. Both lines maintained pluripotency, a normal karyotype, parental cell morphology, and the ability to differentiate into the three germ layers.

Original languageEnglish
Article number102731
JournalStem Cell Research
Volume60
DOIs
StatePublished - Apr 2022
Externally publishedYes

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