Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines

Fangfang Zhang; Anna B. Meier; Karl Ludwig Laugwitz; Tatjana Dorn; Alessandra Moretti

doi:10.1016/j.scr.2021.102612

Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines

Fangfang Zhang
, Anna B. Meier
, Karl Ludwig Laugwitz
, Tatjana Dorn
, Alessandra Moretti

Technical University of Munich
Partner Site Munich Heart Alliance

Research output: Contribution to journal › Article › peer-review

Abstract

Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines – one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) – by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential and a normal karyotype.

Original language	English
Article number	102612
Journal	Stem Cell Research
Volume	57
DOIs	https://doi.org/10.1016/j.scr.2021.102612
State	Published - Dec 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1016/j.scr.2021.102612

Cite this

@article{ce84133e474343b093efa67795e2e3c4,

title = "Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines",

abstract = "Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines – one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) – by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential and a normal karyotype.",

author = "Fangfang Zhang and Meier, \{Anna B.\} and Laugwitz, \{Karl Ludwig\} and Tatjana Dorn and Alessandra Moretti",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = dec,

doi = "10.1016/j.scr.2021.102612",

language = "English",

volume = "57",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines

AU - Zhang, Fangfang

AU - Meier, Anna B.

AU - Laugwitz, Karl Ludwig

AU - Dorn, Tatjana

AU - Moretti, Alessandra

PY - 2021/12

Y1 - 2021/12

N2 - Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines – one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) – by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential and a normal karyotype.

AB - Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines – one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) – by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential and a normal karyotype.

UR - https://www.scopus.com/pages/publications/85120304579

U2 - 10.1016/j.scr.2021.102612

DO - 10.1016/j.scr.2021.102612

M3 - Article

C2 - 34864222

AN - SCOPUS:85120304579

SN - 1873-5061

VL - 57

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102612

ER -

Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this