TY - GEN
T1 - Generation of free-form, dynamic temperature gradients in a disposable microchip
AU - Ha, B. H.
AU - Park, J.
AU - Destgeer, G.
AU - Jung, J. H.
AU - Sung, H. J.
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - We present a temperature gradient (TG) generation method using acoustothermal heating of polydimethylsiloxane (PDMS) by surface acoustic wave (SAW) microfluidic system [1]. Independent and precise control of each SAW from a slanted-finger interdigital transducer (SIDT) enables a onedimensional (1D) spatiotemporal control of temperature in a PDMS microchip. Various TGs were created in solid, gas and liquid. In contrast to prior works [2], the system creates switchable, dynamic and freeform TGs in a disposable microchip. As an application, one-shot high-resolution DNA melting curve analysis was performed to detect single nucleotide polymorphism (SNP).
AB - We present a temperature gradient (TG) generation method using acoustothermal heating of polydimethylsiloxane (PDMS) by surface acoustic wave (SAW) microfluidic system [1]. Independent and precise control of each SAW from a slanted-finger interdigital transducer (SIDT) enables a onedimensional (1D) spatiotemporal control of temperature in a PDMS microchip. Various TGs were created in solid, gas and liquid. In contrast to prior works [2], the system creates switchable, dynamic and freeform TGs in a disposable microchip. As an application, one-shot high-resolution DNA melting curve analysis was performed to detect single nucleotide polymorphism (SNP).
KW - Acoustothermal heating
KW - High-resolution melting
KW - Microfluidics
KW - Polydimethylsiloxane
KW - Slanted-finger IDT
KW - Surface acoustic wave
UR - http://www.scopus.com/inward/record.url?scp=84983339470&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983339470
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1011
EP - 1013
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -