Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation

Eunike Sawitning Ayu Setyono; Nicole Katarina Rogers; Anita Hofmann; Heiko Lickert; Ingo Burtscher

doi:10.1016/j.scr.2025.103685

Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation

Eunike Sawitning Ayu Setyono, Nicole Katarina Rogers, Anita Hofmann, Heiko Lickert, Ingo Burtscher

Department of Pediatric Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.

Original language	English
Article number	103685
Journal	Stem Cell Research
Volume	84
DOIs	https://doi.org/10.1016/j.scr.2025.103685
State	Published - Apr 2025

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title = "Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation",

abstract = "Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.",

author = "Setyono, {Eunike Sawitning Ayu} and Rogers, {Nicole Katarina} and Anita Hofmann and Heiko Lickert and Ingo Burtscher",

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AU - Rogers, Nicole Katarina

AU - Hofmann, Anita

AU - Lickert, Heiko

AU - Burtscher, Ingo

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AB - Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.

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JO - Stem Cell Research

JF - Stem Cell Research

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ER -

Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation

Abstract

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