Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)

Noel Moya; Alireza Shahryari; Ingo Burtscher; Julia Beckenbauer; Mostafa Bakhti; Heiko Lickert

doi:10.1016/j.scr.2020.101874

Generation of a homozygous ARX nuclear CFP (ARX^nCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)

Noel Moya
, Alireza Shahryari
, Ingo Burtscher
, Julia Beckenbauer
, Mostafa Bakhti
, Heiko Lickert

Department of Pediatric Medicine

Helmholtz Zentrum München German Research Center for Environmental Health
University of Munich
Golestan University of Medical Sciences
Technical University of Munich
German Centre for Diabetes Research (DZD)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARX^nCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon⁺ α-like cells. Thus, ARX^nCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX⁺ α-like cell function in vitro.

Original language	English
Article number	101874
Journal	Stem Cell Research
Volume	46
DOIs	https://doi.org/10.1016/j.scr.2020.101874
State	Published - Jul 2020

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title = "Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)",

abstract = "The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARXnCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon+ α-like cells. Thus, ARXnCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX+ α-like cell function in vitro.",

author = "Noel Moya and Alireza Shahryari and Ingo Burtscher and Julia Beckenbauer and Mostafa Bakhti and Heiko Lickert",

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T1 - Generation of a homozygous ARX nuclear CFP (ARXnCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)

AU - Moya, Noel

AU - Shahryari, Alireza

AU - Burtscher, Ingo

AU - Beckenbauer, Julia

AU - Bakhti, Mostafa

AU - Lickert, Heiko

PY - 2020/7

Y1 - 2020/7

N2 - The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARXnCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon+ α-like cells. Thus, ARXnCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX+ α-like cell function in vitro.

AB - The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-localized histone 2B-cyan fluorescent protein (nCFP). The resulting cells have a normal karyotype and preserved pluripotency. In vitro differentiation of the ARXnCFP/nCFP reporter iPSCs towards the endocrine lineage confirmed the specific co-expression of the reporter protein in human glucagon+ α-like cells. Thus, ARXnCFP/nCFP iPSC line will provide a powerful tool to monitor human α-cell progenitor differentiation as well as ARX+ α-like cell function in vitro.

UR - https://www.scopus.com/pages/publications/85086606107

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DO - 10.1016/j.scr.2020.101874

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Generation of a homozygous ARX nuclear CFP (ARX^nCFP/nCFP) reporter human iPSC line (HMGUi001-A-4)

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