Gene-for-gene relationship in the host-pathogen system Malus × robusta 5-Erwinia amylovora

Isabelle Vogt; Thomas Wöhner; Klaus Richter; Henryk Flachowsky; George W. Sundin; Annette Wensing; Elizabeth A. Savory; Klaus Geider; Brad Day; Magda Viola Hanke; Andreas Peil

doi:10.1111/nph.12094

Gene-for-gene relationship in the host-pathogen system Malus × robusta 5-Erwinia amylovora

Isabelle Vogt, Thomas Wöhner, Klaus Richter, Henryk Flachowsky, George W. Sundin, Annette Wensing, Elizabeth A. Savory, Klaus Geider, Brad Day, Magda Viola Hanke, Andreas Peil

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Abstract

Fire blight is a destructive bacterial disease caused by Erwinia amylovora affecting plants in the family Rosaceae, including apple. Host resistance to fire blight is present mainly in accessions of Malus spp. and is thought to be quantitative in this pathosystem. In this study we analyzed the importance of the E. amylovora effector avrRpt2_EA, a homolog of Pseudomonas syringae avrRpt2, for resistance of Malus × robusta 5 (Mr5). The deletion mutant E. amylovora Ea1189ΔavrRpt2_EA was able to overcome the fire blight resistance of Mr5. One single nucleotide polymorphism (SNP), resulting in an exchange of cysteine to serine in the encoded protein, was detected in avrRpt2_EA of several Erwinia strains differing in virulence to Mr5. E. amylovora strains encoding serine (S-allele) were able to overcome resistance of Mr5, whereas strains encoding cysteine (C-allele) were not. Allele specificity was also observed in a coexpression assay with Arabidopsis thaliana RIN4 in Nicotiana benthamiana. A homolog of RIN4 has been detected and isolated in Mr5. These results suggest a system similar to the interaction of RPS2 from A. thaliana and AvrRpt2 from P. syringae with RIN4 as guard. Our data are suggestive of a gene-for-gene relationship for the host-pathogen system Mr5 and E. amylovora.

Original language	English
Pages (from-to)	1262-1275
Number of pages	14
Journal	New Phytologist
Volume	197
Issue number	4
DOIs	https://doi.org/10.1111/nph.12094
State	Published - Mar 2013
Externally published	Yes

Keywords

AvrRpt2
Fire blight
Gene-for-gene interaction
Malus × robusta 5
Resistance

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10.1111/nph.12094

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@article{9dc3cdbd255b44b496cf03489515ab7d,

title = "Gene-for-gene relationship in the host-pathogen system Malus × robusta 5-Erwinia amylovora",

abstract = "Fire blight is a destructive bacterial disease caused by Erwinia amylovora affecting plants in the family Rosaceae, including apple. Host resistance to fire blight is present mainly in accessions of Malus spp. and is thought to be quantitative in this pathosystem. In this study we analyzed the importance of the E. amylovora effector avrRpt2EA, a homolog of Pseudomonas syringae avrRpt2, for resistance of Malus × robusta 5 (Mr5). The deletion mutant E. amylovora Ea1189ΔavrRpt2EA was able to overcome the fire blight resistance of Mr5. One single nucleotide polymorphism (SNP), resulting in an exchange of cysteine to serine in the encoded protein, was detected in avrRpt2EA of several Erwinia strains differing in virulence to Mr5. E. amylovora strains encoding serine (S-allele) were able to overcome resistance of Mr5, whereas strains encoding cysteine (C-allele) were not. Allele specificity was also observed in a coexpression assay with Arabidopsis thaliana RIN4 in Nicotiana benthamiana. A homolog of RIN4 has been detected and isolated in Mr5. These results suggest a system similar to the interaction of RPS2 from A. thaliana and AvrRpt2 from P. syringae with RIN4 as guard. Our data are suggestive of a gene-for-gene relationship for the host-pathogen system Mr5 and E. amylovora.",

keywords = "AvrRpt2, Fire blight, Gene-for-gene interaction, Malus × robusta 5, Resistance",

author = "Isabelle Vogt and Thomas W{\"o}hner and Klaus Richter and Henryk Flachowsky and Sundin, {George W.} and Annette Wensing and Savory, {Elizabeth A.} and Klaus Geider and Brad Day and Hanke, {Magda Viola} and Andreas Peil",

year = "2013",

month = mar,

doi = "10.1111/nph.12094",

language = "English",

volume = "197",

pages = "1262--1275",

journal = "New Phytologist",

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TY - JOUR

T1 - Gene-for-gene relationship in the host-pathogen system Malus × robusta 5-Erwinia amylovora

AU - Vogt, Isabelle

AU - Wöhner, Thomas

AU - Richter, Klaus

AU - Flachowsky, Henryk

AU - Sundin, George W.

AU - Wensing, Annette

AU - Savory, Elizabeth A.

AU - Geider, Klaus

AU - Day, Brad

AU - Hanke, Magda Viola

AU - Peil, Andreas

PY - 2013/3

Y1 - 2013/3

N2 - Fire blight is a destructive bacterial disease caused by Erwinia amylovora affecting plants in the family Rosaceae, including apple. Host resistance to fire blight is present mainly in accessions of Malus spp. and is thought to be quantitative in this pathosystem. In this study we analyzed the importance of the E. amylovora effector avrRpt2EA, a homolog of Pseudomonas syringae avrRpt2, for resistance of Malus × robusta 5 (Mr5). The deletion mutant E. amylovora Ea1189ΔavrRpt2EA was able to overcome the fire blight resistance of Mr5. One single nucleotide polymorphism (SNP), resulting in an exchange of cysteine to serine in the encoded protein, was detected in avrRpt2EA of several Erwinia strains differing in virulence to Mr5. E. amylovora strains encoding serine (S-allele) were able to overcome resistance of Mr5, whereas strains encoding cysteine (C-allele) were not. Allele specificity was also observed in a coexpression assay with Arabidopsis thaliana RIN4 in Nicotiana benthamiana. A homolog of RIN4 has been detected and isolated in Mr5. These results suggest a system similar to the interaction of RPS2 from A. thaliana and AvrRpt2 from P. syringae with RIN4 as guard. Our data are suggestive of a gene-for-gene relationship for the host-pathogen system Mr5 and E. amylovora.

AB - Fire blight is a destructive bacterial disease caused by Erwinia amylovora affecting plants in the family Rosaceae, including apple. Host resistance to fire blight is present mainly in accessions of Malus spp. and is thought to be quantitative in this pathosystem. In this study we analyzed the importance of the E. amylovora effector avrRpt2EA, a homolog of Pseudomonas syringae avrRpt2, for resistance of Malus × robusta 5 (Mr5). The deletion mutant E. amylovora Ea1189ΔavrRpt2EA was able to overcome the fire blight resistance of Mr5. One single nucleotide polymorphism (SNP), resulting in an exchange of cysteine to serine in the encoded protein, was detected in avrRpt2EA of several Erwinia strains differing in virulence to Mr5. E. amylovora strains encoding serine (S-allele) were able to overcome resistance of Mr5, whereas strains encoding cysteine (C-allele) were not. Allele specificity was also observed in a coexpression assay with Arabidopsis thaliana RIN4 in Nicotiana benthamiana. A homolog of RIN4 has been detected and isolated in Mr5. These results suggest a system similar to the interaction of RPS2 from A. thaliana and AvrRpt2 from P. syringae with RIN4 as guard. Our data are suggestive of a gene-for-gene relationship for the host-pathogen system Mr5 and E. amylovora.

KW - AvrRpt2

KW - Fire blight

KW - Gene-for-gene interaction

KW - Malus × robusta 5

KW - Resistance

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U2 - 10.1111/nph.12094

DO - 10.1111/nph.12094

M3 - Article

C2 - 23301854

AN - SCOPUS:84873275094

SN - 0028-646X

VL - 197

SP - 1262

EP - 1275

JO - New Phytologist

JF - New Phytologist

IS - 4

ER -

Gene-for-gene relationship in the host-pathogen system Malus × robusta 5-Erwinia amylovora

Abstract

Keywords

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