G-protein beta-3-subunit and eNOS gene polymorphism in transplant recipients with long-term renal graft function

Background: Despite new immunosuppressive drugs, only a minority of graft survive over 15 years. The aim of our study was to determine the influence of gene polymorphisms in the G-protein-β₃ subunit (Gβ3) and endothelial nitric oxide synthase (eNOS) on the long-term outcome of kidney grafts. Methods: Using PCR, corresponding genotypes in Gβ3 (C825T) and eNOS (G894T) gene polymorphism were evaluated in patients with preserved graft function over 15 years and in a control group of transplant recipients. Results: There were no differences in allele and genotype distributions of both polymorphisms between groups. In Gβ3 polymorphism, the 825T allele carriers had a significantly lower body mass index while in eNOS polymorphism there were no links between genotypes, renal function and atherosclerosis risk factors. Conclusions: Our data suggest that these gene polymorphisms have only a minor influence on long-term renal graft function.