@inproceedings{f93e4938cf084a2fb0ac8bfda00b3e2b,
title = "Functional live-cell mid-infrared microscopy and spectroscopy by optoacoustic and optothermal detection",
abstract = "Mid-infrared (mid-IR) spectroscopy and optoacoustic/optothermal (OA/OT) imaging are perfectly complementary technologies to each other. Vibrational molecular excitations by mid-IR absorption are utterly de-excited in the form of heat while efficient OA/OT signal generation primarily depends on heat deposition. This synergy allows overcoming the (otherwise) persistent limitations of traditional mid-IR spectroscopy and imaging in live-cell/fresh-tissue applications—i.e., sample opacity due to water absorption. Combination of mid-IR excitation and OA/OT detection has resulted in new tools for label-free live-cell, tissues, and in vivo metabolic research. Here we discuss basic principles on mid-IR detection for spectroscopy and imaging as well as the most recent developments on mid-IR OA and OT microscopy that overcome the limitations of conventional vibrational spectroscopy for biosensing and label-free metabolic microscopy.",
keywords = "label-free chemical microscopy, live-cell mid-infrared microscopy, metabolic imaging, optoacoustic/optothermal detection, positive contrast detection",
author = "Tao Yuan and Francesca Gasparin and Nasire Uluc and Vasilis Ntziachristos and Pleitez, {Miguel A.}",
note = "Publisher Copyright: {\textcopyright} 2023 SPIE.; Advanced Chemical Microscopy for Life Science and Translational Medicine 2023 ; Conference date: 28-01-2023 Through 30-01-2023",
year = "2023",
doi = "10.1117/12.2647779",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Ji-Xin Cheng and Wei Min and Simpson, {Garth J.}",
booktitle = "Advanced Chemical Microscopy for Life Science and Translational Medicine 2023",
}