Functional live-cell mid-infrared microscopy and spectroscopy by optoacoustic and optothermal detection

Tao Yuan, Francesca Gasparin, Nasire Uluc, Vasilis Ntziachristos, Miguel A. Pleitez

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Mid-infrared (mid-IR) spectroscopy and optoacoustic/optothermal (OA/OT) imaging are perfectly complementary technologies to each other. Vibrational molecular excitations by mid-IR absorption are utterly de-excited in the form of heat while efficient OA/OT signal generation primarily depends on heat deposition. This synergy allows overcoming the (otherwise) persistent limitations of traditional mid-IR spectroscopy and imaging in live-cell/fresh-tissue applications—i.e., sample opacity due to water absorption. Combination of mid-IR excitation and OA/OT detection has resulted in new tools for label-free live-cell, tissues, and in vivo metabolic research. Here we discuss basic principles on mid-IR detection for spectroscopy and imaging as well as the most recent developments on mid-IR OA and OT microscopy that overcome the limitations of conventional vibrational spectroscopy for biosensing and label-free metabolic microscopy.

Original languageEnglish
Title of host publicationAdvanced Chemical Microscopy for Life Science and Translational Medicine 2023
EditorsJi-Xin Cheng, Wei Min, Garth J. Simpson
PublisherSPIE
ISBN (Electronic)9781510658899
DOIs
StatePublished - 2023
EventAdvanced Chemical Microscopy for Life Science and Translational Medicine 2023 - San Francisco, United States
Duration: 28 Jan 202330 Jan 2023

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume12392
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Chemical Microscopy for Life Science and Translational Medicine 2023
Country/TerritoryUnited States
CitySan Francisco
Period28/01/2330/01/23

Keywords

  • label-free chemical microscopy
  • live-cell mid-infrared microscopy
  • metabolic imaging
  • optoacoustic/optothermal detection
  • positive contrast detection

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