Function of DNA Polymerase I in Rna-Primed Synthesis of Bacteriophage M-13 Duplex DNA

Peter K. Schneck, Walter L. Staudenbauer, Peter Hans Hofschneider

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Cell-free extracts from Escherichia coli contain a DNA polymerase activity resistant to SH-blocking agents, which is capable of synthesizing complementary strand DNA on a circular M-13 DNA template by extension of RNA primers. This activity is considered to be identical with DNA polymerase I (or some altered form of this enzyme) since it is missing in extracts from polA cells. DNA synthesis in the presence of SH-blocking agents occurs at a reduced rate as compared to untreated controls and leads to the formation of DNA chains of defined size (0.4-0.5 genome’s length). It is concluded that efficient M-13 duplex DNA synthesis requires the cooperation of both DNA polymerase I and III.

Original languageEnglish
Pages (from-to)599-614
Number of pages16
JournalNucleic Acids Research
Volume3
Issue number3
DOIs
StatePublished - Mar 1976
Externally publishedYes

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