Abstract
Cell-free extracts from Escherichia coli contain a DNA polymerase activity resistant to SH-blocking agents, which is capable of synthesizing complementary strand DNA on a circular M-13 DNA template by extension of RNA primers. This activity is considered to be identical with DNA polymerase I (or some altered form of this enzyme) since it is missing in extracts from polA cells. DNA synthesis in the presence of SH-blocking agents occurs at a reduced rate as compared to untreated controls and leads to the formation of DNA chains of defined size (0.4-0.5 genome’s length). It is concluded that efficient M-13 duplex DNA synthesis requires the cooperation of both DNA polymerase I and III.
Original language | English |
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Pages (from-to) | 599-614 |
Number of pages | 16 |
Journal | Nucleic Acids Research |
Volume | 3 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1976 |
Externally published | Yes |