Foxp3 expression in pancreatic carcinoma cells as a novel mechanism of immune evasion in cancer

Sebastian Hinz, Laia Pagerols-Raluy, Hans Heinrich Oberg, Ole Ammerpohl, Sandra Grüssel, Bence Sipos, Robert Grützmann, Christian Pilarsky, Hendrik Ungefroren, Hans Detlev Saeger, Günter Klöppel, Dieter Kabelitz, Holger Kalthoff

Research output: Contribution to journalArticlepeer-review

310 Scopus citations

Abstract

The forkhead transcription factor Foxp3 is highly expressed in CD4+CD25+ regulatory T cells (Treg) and was recently identified as a key player in mediating their inhibitory functions. Here, we describe for the first time the expression and function of Foxp3 in pancreatic ductal adenocarcinoma cells and tumors. Foxp3 expression was induced by transforming growth factor-β2 (TGF-β2), but not TGF-β1 stimulation in these cells, and was partially suppressed following antibody-mediated neutralization of TGF-β2. The TGF-β2 effect could be mimicked by ectopic expression of a constitutively active TGF-β type I receptor/ALK5 mutant. Down-regulation of Foxp3 with small interfering RNA (siRNA) in pancreatic carcinoma cells resulted in the up-regulation of interleukin 6 (IL-6) and IL-8 expression, providing evidence for a negative transcriptional activity of Foxp3 also in these epithelial cells. Coculture of Foxp3-expressing tumor cells with naive T cells completely inhibited T-cell proliferation, but not activation, and this antiproliferative effect was partially abrogated following specific inhibition of Foxp3 expression. These findings indicate that pancreatic carcinoma cells share growth-suppressive effects with Treg and suggest that mimicking Treg function may represent a new mechanism of immune evasion in pancreatic cancer.

Original languageEnglish
Pages (from-to)8344-8350
Number of pages7
JournalCancer Research
Volume67
Issue number17
DOIs
StatePublished - 1 Sep 2007
Externally publishedYes

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