Formation of 6’-Deoxychalcone 4’-Glucosides by Enzyme Extracts from Petals of Dahlia variabilis

Karl Stich, Heidrun Halbwirth, Friedrich Wurst, Gert Forkmann

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Yellow colouration of Dahlia variabilis is mainly provided by isoliquiritigenin 4’-glucoside and butein 4’-glucoside. Incubation of petal extracts with uridin 5’-diphosphoglucose and isoliquiritigenin or butein led to the formation of one product which was identified as the respective 6’-deoxychalcone 4’-glucoside. Glucosylation of hydroxyl groups in other positions was not observed. Naringenin chalcone and eriodictyol chalcone were not accepted as substrates. The 4’-glucosylation of isoliquiritigenin and butein showed a broad pH optimum ranging from pH 7 to 8 and was stimulated by Mg2+, Ca2+ and Mn2+. N-Ethylmaleimide, p-hydroxymercuribenzoate, Cu2+, Zn2+, and Fe2+ clearly reduced the activity of the enzyme. The apparent Kmvalues for UDP-glucose, isoliquiritigenin and butein were 90, 26 and 276 µM respectively.

Original languageEnglish
Pages (from-to)737-741
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume49
Issue number11-12
DOIs
StatePublished - Dec 1994

Keywords

  • 4’-O-Glucosyltransferase
  • 6’-Deoxychalcone
  • Butein
  • Chalcone
  • Dahlia variabilis
  • Isoliquirtigenin
  • UDP-Glucose

Fingerprint

Dive into the research topics of 'Formation of 6’-Deoxychalcone 4’-Glucosides by Enzyme Extracts from Petals of Dahlia variabilis'. Together they form a unique fingerprint.

Cite this