TY - JOUR
T1 - Force-induced destabilization of focal adhesions at defined integrin spacings on nanostructured surfaces
AU - De Beer, Alex G.F.
AU - Cavalcanti-Adam, E. Ada
AU - Majer, Günter
AU - Lopez-García, M.
AU - Kessler, H.
AU - Spatz, Joachim P.
PY - 2010/5/12
Y1 - 2010/5/12
N2 - Focal adhesions are the anchoring points of cells to surfaces and are responsible for a large number of surface sensing processes. Nanopatterning studies have shown physiological changes in fibroblasts as a result of decreasing density of external binding ligands. The most striking of these changes is a decreased ability to form mature focal adhesions when lateral ligand distances exceed 76 nm. These changes are usually examined in the context of protein signaling and protein interactions. We show a physical explanation based on the balance between the forces acting on individual ligand connections and the reaction kinetics of those ligands. We propose three stability regimes for focal adhesions as a function of ligand spacing and applied stress: a stable regime, an unstable regime in which a large fraction of unbound protein causes adhesion disintegration, and a regime in which the applied force is too high to form an adhesion structure.
AB - Focal adhesions are the anchoring points of cells to surfaces and are responsible for a large number of surface sensing processes. Nanopatterning studies have shown physiological changes in fibroblasts as a result of decreasing density of external binding ligands. The most striking of these changes is a decreased ability to form mature focal adhesions when lateral ligand distances exceed 76 nm. These changes are usually examined in the context of protein signaling and protein interactions. We show a physical explanation based on the balance between the forces acting on individual ligand connections and the reaction kinetics of those ligands. We propose three stability regimes for focal adhesions as a function of ligand spacing and applied stress: a stable regime, an unstable regime in which a large fraction of unbound protein causes adhesion disintegration, and a regime in which the applied force is too high to form an adhesion structure.
UR - http://www.scopus.com/inward/record.url?scp=77952418052&partnerID=8YFLogxK
U2 - 10.1103/PhysRevE.81.051914
DO - 10.1103/PhysRevE.81.051914
M3 - Article
AN - SCOPUS:77952418052
SN - 1539-3755
VL - 81
JO - Physical Review E - Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics
JF - Physical Review E - Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics
IS - 5
M1 - 051914
ER -