Fluorescence immunohistochemical detection of Armillaria and Heterobasidion in Norway spruce

U. Zollfrank; C. Sautter; B. Hock

doi:10.1111/j.1439-0329.1987.tb01021.x

Fluorescence immunohistochemical detection of Armillaria and Heterobasidion in Norway spruce

U. Zollfrank, C. Sautter, B. Hock

Life Sciences

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Armillaria mellea ss. lato and Heterobasidion annosum were identified in infected Norway spruce using an indirect immunohistochemical test. Unspecific binding sites were masked by incubating the specimen with preimmune serum followed by protein A. Subsequent labeling with monospecific rabbit antibodies and fluorochrome‐conjugated protein A yielded a strong and specific fluorescent signal.

Original language	English
Pages (from-to)	230-237
Number of pages	8
Journal	European Journal of Forest Pathology
Volume	17
Issue number	4-5
DOIs	https://doi.org/10.1111/j.1439-0329.1987.tb01021.x
State	Published - Aug 1987

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title = "Fluorescence immunohistochemical detection of Armillaria and Heterobasidion in Norway spruce",

abstract = "Armillaria mellea ss. lato and Heterobasidion annosum were identified in infected Norway spruce using an indirect immunohistochemical test. Unspecific binding sites were masked by incubating the specimen with preimmune serum followed by protein A. Subsequent labeling with monospecific rabbit antibodies and fluorochrome‐conjugated protein A yielded a strong and specific fluorescent signal.",

author = "U. Zollfrank and C. Sautter and B. Hock",

year = "1987",

month = aug,

doi = "10.1111/j.1439-0329.1987.tb01021.x",

language = "English",

volume = "17",

pages = "230--237",

journal = "European Journal of Forest Pathology",

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publisher = "Wiley-Blackwell Publishing Ltd",

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T1 - Fluorescence immunohistochemical detection of Armillaria and Heterobasidion in Norway spruce

AU - Zollfrank, U.

AU - Sautter, C.

AU - Hock, B.

PY - 1987/8

Y1 - 1987/8

N2 - Armillaria mellea ss. lato and Heterobasidion annosum were identified in infected Norway spruce using an indirect immunohistochemical test. Unspecific binding sites were masked by incubating the specimen with preimmune serum followed by protein A. Subsequent labeling with monospecific rabbit antibodies and fluorochrome‐conjugated protein A yielded a strong and specific fluorescent signal.

AB - Armillaria mellea ss. lato and Heterobasidion annosum were identified in infected Norway spruce using an indirect immunohistochemical test. Unspecific binding sites were masked by incubating the specimen with preimmune serum followed by protein A. Subsequent labeling with monospecific rabbit antibodies and fluorochrome‐conjugated protein A yielded a strong and specific fluorescent signal.

UR - http://www.scopus.com/inward/record.url?scp=85005301867&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0329.1987.tb01021.x

DO - 10.1111/j.1439-0329.1987.tb01021.x

M3 - Article

AN - SCOPUS:85005301867

SN - 0300-1237

VL - 17

SP - 230

EP - 237

JO - European Journal of Forest Pathology

JF - European Journal of Forest Pathology

IS - 4-5

ER -

Fluorescence immunohistochemical detection of Armillaria and Heterobasidion in Norway spruce

Abstract

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