Fixed archival tissue: Purify DNA and primers for good PCR yield!

Gregor Weirich; Maria Anna Hornauer; Thomas Brüning; Heinz Höfler; Hiltrud Brauch

doi:10.1007/BF02760783

Fixed archival tissue: Purify DNA and primers for good PCR yield!

Gregor Weirich
, Maria Anna Hornauer
, Thomas Brüning
, Heinz Höfler
, Hiltrud Brauch

TUM Emeriti of Excellence

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.

Original language	English
Pages (from-to)	299-301
Number of pages	3
Journal	Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume	8
Issue number	3
DOIs	https://doi.org/10.1007/BF02760783
State	Published - Dec 1997

Keywords

DNA-extraction
Fixation
PCR-HPLC

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10.1007/BF02760783

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abstract = "The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.",

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T2 - Purify DNA and primers for good PCR yield!

AU - Weirich, Gregor

AU - Hornauer, Maria Anna

AU - Brüning, Thomas

AU - Höfler, Heinz

AU - Brauch, Hiltrud

PY - 1997/12

Y1 - 1997/12

N2 - The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.

AB - The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.

KW - DNA-extraction

KW - Fixation

KW - PCR-HPLC

UR - https://www.scopus.com/pages/publications/0031309554

U2 - 10.1007/BF02760783

DO - 10.1007/BF02760783

M3 - Article

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AN - SCOPUS:0031309554

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JO - Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

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IS - 3

ER -

Fixed archival tissue: Purify DNA and primers for good PCR yield!

Abstract

Keywords

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