Fixed archival tissue: Purify DNA and primers for good PCR yield!

Gregor Weirich, Maria Anna Hornauer, Thomas Brüning, Heinz Höfler, Hiltrud Brauch

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.

Original languageEnglish
Pages (from-to)299-301
Number of pages3
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Issue number3
StatePublished - Dec 1997


  • DNA-extraction
  • Fixation


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