TY - JOUR
T1 - Fixed archival tissue
T2 - Purify DNA and primers for good PCR yield!
AU - Weirich, Gregor
AU - Hornauer, Maria Anna
AU - Brüning, Thomas
AU - Höfler, Heinz
AU - Brauch, Hiltrud
PY - 1997/12
Y1 - 1997/12
N2 - The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.
AB - The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.
KW - DNA-extraction
KW - Fixation
KW - PCR-HPLC
UR - http://www.scopus.com/inward/record.url?scp=0031309554&partnerID=8YFLogxK
U2 - 10.1007/BF02760783
DO - 10.1007/BF02760783
M3 - Article
C2 - 9438264
AN - SCOPUS:0031309554
SN - 1073-6085
VL - 8
SP - 299
EP - 301
JO - Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology
JF - Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology
IS - 3
ER -