@article{ebfff792412d45daaccc64f56bdf8728,
title = "First results of GEN-AU: Cloning of Deoxynivalenol- and Zearalenoneinactivating U DP-g lucosyltransferase genes from Arabidopsis thaliana and expression in yeast for production of mycotoxin-glucosides",
abstract = "First results of the GEN-AU pilot project {"}Fusarium virulence and plant resistance mechanisms{"} are reported. Employing genetically engineered yeast strains we have been able to clone genes from the model plant Arabidopsis thaliana encoding UDP-glucosyltransferases which can inactivate deoxynivalenol (DON) and zearalenone (ZON). The structure of the metabolites produced by the transformed yeast strains were determined by LC-MS/MS as DON-30-glucoside and ZON-40- glucoside, respectively. ZON and derivatives added to glucosyltransferase expressing yeast cultures are converted into the corresponding glucosides in very high yield, opening an efficient way to produce reference materials for these masked mycotoxins.",
keywords = "Deoxynivalenol-glucoside, Zearalenone-glucoside",
author = "B. Poppenberger and F. Berthiller and D. Lucyshyn and R. Schuhmacher and R. Krska and G. Adam",
note = "Funding Information: see: http://www.gen-au.at). A GEN-AU pilot project is addressing the Fusarium problem in an interdisciplinary approach, utilizing the new tools of genome research. Starting point of the project is the full genome sequence of Fusarium graminearum (Gibberella zeae), which has been determined with public funding from the US National Research Initiative (USDA/NSF Microbial Genome Sequencing Project) at the Broad Institute. (Fusarium graminearum Database, see: http://www.broad. mit.edu/annotatiordfungi/fusarium/). Since gene prediction in fungi is nontrivial, and in order to provide access to the vast amount of bioinfor-matical predictions, part of the funding of the GEN-AU pilot project was used to fund the establishment of a user friendly database by MIPS (GSF, Munich Information Center on Protein Sequences: http://mips.gsf.de/genre/ proj/fusarium/).",
year = "2005",
month = jun,
doi = "10.1007/BF02954431",
language = "English",
volume = "21",
pages = "108--111",
journal = "Mycotoxin Research",
issn = "0178-7888",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "2",
}