Fibroblast growth factor induces primitive streak formation in rabbit pre-implantation embryos in vitro

M. Hrabě de Angelis, C. Kirchner

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Culturing of rabbit pre-implantation embryos was performed in Ham's F10 medium supplemented with polyvinylpyrrolidone. Under these culture conditions, day 6 post coitum blastocysts increased their diameter within 24 h to 80% of that of day 7 blastocysts grown in vivo. Despite this substained growth, the embryonic disc remained undifferentiated with clear signs of degeneration after 24 h of culture. Basic fibroblast growth factor (bFGF) was able to overcome this developmental block. After 12 h of culture, day 6 blastocysts showed pear-shaped embryonic discs, and after 24 h, the primitive streak with Hensen's node was visible. The bFGF had no comparable effects on day 5 and day 7 blastocysts. The embryonic discs of day 5 blastocysts degenerated, even in the presence of bFGF, whereas day 7 blastocysts were able to form their primitive streak, also in the absence of bFGF. TGF β1 did not promote embryonic development in vitro. The data indicate that the onset of mesoderm formation in the rabbit is controlled by a growth factor of the FGF-family.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalAnatomy and Embryology
Volume187
Issue number3
DOIs
StatePublished - Mar 1993
Externally publishedYes

Keywords

  • Blastocyst
  • Fibroblast growth factor
  • In vitro culture
  • Mesoderm-inducing factor
  • Rabbit

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