TY - JOUR
T1 - Fibroblast and Vascular Endothelial Growth Factor Coating of Decellularized Vascular Grafts Stimulates Undesired Giant Cells and Graft Encapsulation in a Rat Model
AU - Heidenhain, Christoph
AU - Veeravoorn, Ariyakhagorn
AU - Vachkov, Blagovest
AU - Weichert, Wilko
AU - Schmidmaier, Gerhard
AU - Wildemann, Britt
AU - Neuhaus, Peter
AU - Heise, Michael
PY - 2011/1
Y1 - 2011/1
N2 - Replacing an infected prosthesis with a bioimplant provides a hopeful alternative in septic vascular surgery. The objective of this study was to determine the effect of fibroblast endothelial growth factors (FGF) and vascular endothelial growth factors (VEGF) coating on a decellularized vascular graft in a rat model and the possible impact on recellularization processes. Rat aortas were decellularized, crosslinked with genipin, and coated with poly-(D, L) lactide containing either FGF or VEGF. Observation periods were 6 and 12 weeks. Surprisingly, we found moderate accumulation of giant cells around the grafts that contained poly-(D, L) lactide acid. FGF and VEGF grafts showed massive stimulation of giant cells and eosinophils leading to complete graft encapsulation (P < 0.05). Pseudointmal hyperplasia was significantly increased in the FGF group (P < 0.05). Both results can only be interpreted as very negative. We achieved a situation in diametric opposition to that which we had hoped for. These data demonstrate that the use of growth factors may produce harmful side effects.
AB - Replacing an infected prosthesis with a bioimplant provides a hopeful alternative in septic vascular surgery. The objective of this study was to determine the effect of fibroblast endothelial growth factors (FGF) and vascular endothelial growth factors (VEGF) coating on a decellularized vascular graft in a rat model and the possible impact on recellularization processes. Rat aortas were decellularized, crosslinked with genipin, and coated with poly-(D, L) lactide containing either FGF or VEGF. Observation periods were 6 and 12 weeks. Surprisingly, we found moderate accumulation of giant cells around the grafts that contained poly-(D, L) lactide acid. FGF and VEGF grafts showed massive stimulation of giant cells and eosinophils leading to complete graft encapsulation (P < 0.05). Pseudointmal hyperplasia was significantly increased in the FGF group (P < 0.05). Both results can only be interpreted as very negative. We achieved a situation in diametric opposition to that which we had hoped for. These data demonstrate that the use of growth factors may produce harmful side effects.
KW - Encapsulation
KW - Fibroblast growth factor
KW - Giant cells
KW - Vascular endothelial growth factor
KW - Vascular grafts
UR - http://www.scopus.com/inward/record.url?scp=78651418247&partnerID=8YFLogxK
U2 - 10.1111/j.1525-1594.2010.01072.x
DO - 10.1111/j.1525-1594.2010.01072.x
M3 - Article
C2 - 20883449
AN - SCOPUS:78651418247
SN - 0160-564X
VL - 35
SP - E1-E10
JO - Artificial Organs
JF - Artificial Organs
IS - 1
ER -