Fed-batch production of a soluble β-1,4-galactosyltransferase with Saccharomyces cerevisiae

Jana Zigovaa, Matthias Mahlea, Holger Pascholda, Martine Malissard, Eric G. Berger, Dirk Weuster-Botz

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A feedback-controlled fed-batch process for the recombinant production of a soluble human β-1,4-galactosyltransferase (NdrGal-T) with Saccharomyces cerevisiae was developed and scaled up to the pilot scale. A 5000 U NdrGal-T fermentation run was performed on a 300 l scale. Indirect feedback control of the glucose feeding with RQ data at a set-point of RQ = 1.1 resulted in higher NdrGal-T activities (30 U/l) than direct feedback control of glucose with on-line flow-injection analysis (9 U/l). This increase in final activity of NdrGal-T by a factor of 42 compared to published data makes the fed-batch production of glycosyltransferases with a Saccharomyces cerevisiae expression system competitive with cell-culture systems. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalEnzyme and Microbial Technology
Volume25
Issue number3-5
DOIs
StatePublished - Aug 1999
Externally publishedYes

Keywords

  • Feedback control
  • Flow-injection analysis
  • Glycosyltransferase
  • Kalman filter
  • Respiratory quotient (RQ)
  • Saccharomyces cerevisiae

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