TY - JOUR
T1 - Expression of stem cell markers and transcription factors during the remodeling of the rat pancreas after duct ligation
AU - Peters, Katharina
AU - Panienka, Roswitha
AU - Li, Jinming
AU - Klöppel, Günter
AU - Wang, Rennian
N1 - Funding Information:
Acknowledgements This work was supported by grants from the Deutsche Forschungsgemeinschaft (PE 732/2–1) and SJHC Foundation Pearl Laird Fund Canada. Dr. Wang is supported by a University Faculty Award of Natural Science and Engineering Research Council of Canada (NSERC).
PY - 2005/1
Y1 - 2005/1
N2 - Ligation of the pancreatic duct has been shown to induce islet cell neogenesis from duct cells in the adult rat pancreas. The transcription factors that regulate islet cell neogenesis and the phenotype of putative precursor cells involved in neogenesis are unknown. We, therefore, studied the expression of the transcription factors Pdx1, Pbx1, Meis2, Nkx2.2 and the putative stem cell markers c-Kit and nestin in rat pancreata 3, 5 and 7 days after duct ligation. Immunocytochemical staining revealed a subpopulation of cells in the ligated portion of the pancreas that was positive for the putative stem cell markers c-Kit and nestin. The c-Kit immunoreactivity was upregulated, reaching a peak at day 3, while nestin expression peaked at day 7. The c-Kit-positive cells were located among the duct and islet cells, while nestin-expressing cells were found scattered in the duct epithelium at day 3 and around the ducts at day 7. Both c-Kit- and nestin-positive cells showed high proliferative activity, as determined by BrdU labeling. Pdx1 and Nkx2.2 were found predominantly in the duct cells of the ligated pancreas. There were significant changes in the expression patterns of Pbx1 and Meis2 in the ductular complexes. The findings indicate that the stem cell markers c-Kit and nestin as well as the transcription factors Pdx1 and Nkx2.2 are upregulated in compartments of the pancreas that are involved in islet cell neogenesis after duct ligation.
AB - Ligation of the pancreatic duct has been shown to induce islet cell neogenesis from duct cells in the adult rat pancreas. The transcription factors that regulate islet cell neogenesis and the phenotype of putative precursor cells involved in neogenesis are unknown. We, therefore, studied the expression of the transcription factors Pdx1, Pbx1, Meis2, Nkx2.2 and the putative stem cell markers c-Kit and nestin in rat pancreata 3, 5 and 7 days after duct ligation. Immunocytochemical staining revealed a subpopulation of cells in the ligated portion of the pancreas that was positive for the putative stem cell markers c-Kit and nestin. The c-Kit immunoreactivity was upregulated, reaching a peak at day 3, while nestin expression peaked at day 7. The c-Kit-positive cells were located among the duct and islet cells, while nestin-expressing cells were found scattered in the duct epithelium at day 3 and around the ducts at day 7. Both c-Kit- and nestin-positive cells showed high proliferative activity, as determined by BrdU labeling. Pdx1 and Nkx2.2 were found predominantly in the duct cells of the ligated pancreas. There were significant changes in the expression patterns of Pbx1 and Meis2 in the ductular complexes. The findings indicate that the stem cell markers c-Kit and nestin as well as the transcription factors Pdx1 and Nkx2.2 are upregulated in compartments of the pancreas that are involved in islet cell neogenesis after duct ligation.
KW - Islet cell neogenesis
KW - Islet progenitor cell markers
KW - Pancreatic duct ligation
KW - Transcription factors
UR - http://www.scopus.com/inward/record.url?scp=13144265752&partnerID=8YFLogxK
U2 - 10.1007/s00428-004-1145-7
DO - 10.1007/s00428-004-1145-7
M3 - Article
C2 - 15660282
AN - SCOPUS:13144265752
SN - 0945-6317
VL - 446
SP - 56
EP - 63
JO - Virchows Archiv
JF - Virchows Archiv
IS - 1
ER -