Expression of Lactobacillus casei ATCC 393 β‐galactosidase encoded by plasmid pLZ15 in Lactococcus lactis CNRZ 1123

D. Hemme, W. Gaier, D. A. Winters, Catherine Foucaud, R. F. Vogel

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Abstract

Lactococcus lactis subsp. lactis CNRZ 1123, a Lac derivative of CNRZ 1122 was transformed by electroporation with the Lactobacillus casei ATCC 393 plasmid pLZ15, which bears a β‐galactosidase gene. The transformants expressed a constitutive β‐galactosidase activity at a higher level than in Lact. casei, and in the cell‐free extract two additional protein bands were detected by SDS‐PAGE which could correspond to lactose metabolism enzymes. Both plasmid and β‐gal activity were stable in Lactococcus after 100 generations in glucose‐containing medium.

Original languageEnglish
Pages (from-to)345-348
Number of pages4
JournalLetters in Applied Microbiology
Volume19
Issue number5
DOIs
StatePublished - Nov 1994
Externally publishedYes

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