Abstract
The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-α) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-α was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-α was purified and used for the production of neutralizing antisera. (C) 2000 Academic Press.
Original language | English |
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Pages (from-to) | 573-577 |
Number of pages | 5 |
Journal | Cytokine |
Volume | 12 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2000 |
Externally published | Yes |
Keywords
- Cytokine
- HBV
- TNF-α
- WHV
- Woodchuck