Expression and purification of woodchuck tumour necrosis factor alpha

B. Lohrengel; M. Lu; D. Bauer; M. Roggendorf

doi:10.1006/cyto.1999.0636

Expression and purification of woodchuck tumour necrosis factor alpha

B. Lohrengel, M. Lu, D. Bauer, M. Roggendorf

University Hospital of Essen

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-α) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-α was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-α was purified and used for the production of neutralizing antisera. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	573-577
Number of pages	5
Journal	Cytokine
Volume	12
Issue number	6
DOIs	https://doi.org/10.1006/cyto.1999.0636
State	Published - Jun 2000
Externally published	Yes

Keywords

Cytokine
HBV
TNF-α
WHV
Woodchuck

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10.1006/cyto.1999.0636

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title = "Expression and purification of woodchuck tumour necrosis factor alpha",

abstract = "The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-α) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-α was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-α was purified and used for the production of neutralizing antisera. (C) 2000 Academic Press.",

keywords = "Cytokine, HBV, TNF-α, WHV, Woodchuck",

author = "B. Lohrengel and M. Lu and D. Bauer and M. Roggendorf",

note = "Funding Information: We thank Thekla Kemper for excellent technical assistance. This work was supported by DFG grant RO687/6-1 M.R. and H.L. The work of BL was in part supported by Glaxo Wellcome.",

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T1 - Expression and purification of woodchuck tumour necrosis factor alpha

AU - Lohrengel, B.

AU - Lu, M.

AU - Bauer, D.

AU - Roggendorf, M.

N1 - Funding Information: We thank Thekla Kemper for excellent technical assistance. This work was supported by DFG grant RO687/6-1 M.R. and H.L. The work of BL was in part supported by Glaxo Wellcome.

PY - 2000/6

Y1 - 2000/6

N2 - The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-α) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-α was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-α was purified and used for the production of neutralizing antisera. (C) 2000 Academic Press.

AB - The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-α) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-α was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-α was purified and used for the production of neutralizing antisera. (C) 2000 Academic Press.

KW - Cytokine

KW - HBV

KW - TNF-α

KW - WHV

KW - Woodchuck

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DO - 10.1006/cyto.1999.0636

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SN - 1043-4666

VL - 12

SP - 573

EP - 577

JO - Cytokine

JF - Cytokine

IS - 6

ER -

Expression and purification of woodchuck tumour necrosis factor alpha

Abstract

Keywords

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