TY - JOUR
T1 - Experimental characterization and modelling of analytical monolithic column
AU - Zabka, Michal
AU - Minceva, Mirjana
AU - Rodrigues, Alírio E.
N1 - Funding Information:
Michal Zabka (SFRH/BD/5418/2001) gratefully acknowledges the PhD grant from the “Fundação para a Ciência e Tecnologia”.
PY - 2007/2/23
Y1 - 2007/2/23
N2 - Hydrodynamics, equilibrium and kinetics of adsorption in a silica-based monolithic column Chromolith Performance RP-18e (Merck KgaA, Germany) have been studied. The column permeability was calculated according to the Darcy law for laminar flow. The efficiency of the monolithic column was characterized through the height equivalent to a theoretical plate (HETP) for myoglobin, phenol and progesterone. The 2-D single channel mathematical model has been applied to describe the adsorption dynamics. Parabolic velocity profile, axial and radial diffusion in the monolith channel, linear driving force model for the mass transfer in the monolith channel skeleton wall and linear adsorption equilibrium were assumed. The mathematical model gives good prediction of the experimental elution peaks.
AB - Hydrodynamics, equilibrium and kinetics of adsorption in a silica-based monolithic column Chromolith Performance RP-18e (Merck KgaA, Germany) have been studied. The column permeability was calculated according to the Darcy law for laminar flow. The efficiency of the monolithic column was characterized through the height equivalent to a theoretical plate (HETP) for myoglobin, phenol and progesterone. The 2-D single channel mathematical model has been applied to describe the adsorption dynamics. Parabolic velocity profile, axial and radial diffusion in the monolith channel, linear driving force model for the mass transfer in the monolith channel skeleton wall and linear adsorption equilibrium were assumed. The mathematical model gives good prediction of the experimental elution peaks.
KW - Adsorption of myoglobin
KW - Hydrodynamic characterization
KW - Liquid chromatography
KW - Mathematical modeling
KW - Phenol and progesterone
KW - Silica monolithic column
UR - http://www.scopus.com/inward/record.url?scp=33846879600&partnerID=8YFLogxK
U2 - 10.1016/j.jbbm.2006.10.002
DO - 10.1016/j.jbbm.2006.10.002
M3 - Article
C2 - 17123626
AN - SCOPUS:33846879600
SN - 0165-022X
VL - 70
SP - 95
EP - 105
JO - Journal of Biochemical and Biophysical Methods
JF - Journal of Biochemical and Biophysical Methods
IS - 1
ER -