Expanding the genetic code of Drosophila melanogaster

Ambra Bianco; Fiona M. Townsley; Sebastian Greiss; Kathrin Lang; Jason W. Chin

doi:10.1038/nchembio.1043

Expanding the genetic code of Drosophila melanogaster

Ambra Bianco
, Fiona M. Townsley
, Sebastian Greiss
, Kathrin Lang
, Jason W. Chin

Cambridge Biomedical Campus

Research output: Contribution to journal › Article › peer-review

174 Scopus citations

Abstract

Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.

Original language	English
Pages (from-to)	748-750
Number of pages	3
Journal	Nature Chemical Biology
Volume	8
Issue number	9
DOIs	https://doi.org/10.1038/nchembio.1043
State	Published - Sep 2012
Externally published	Yes

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10.1038/nchembio.1043

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title = "Expanding the genetic code of Drosophila melanogaster",

abstract = "Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.",

author = "Ambra Bianco and Townsley, \{Fiona M.\} and Sebastian Greiss and Kathrin Lang and Chin, \{Jason W.\}",

note = "Funding Information: This work was funded by the UK Medical Research Council (MRC) (grants U105181009 and UD99999908) and the Louis-Jeantet Foundation Young Investigator Career Award (both to J.W.C.). We are grateful to S. Bullock (MRC Laboratory of Molecular Biology) for providing initial plasmids and for extensive help and advice with this project; to S. Peak-Chew and M. Skehel in the MRC Laboratory of Molecular Biology Mass Spectrometry Facility for MS; S. Brunner for assistance with statistical analysis; and J. Schmitz and R. Pisa for experimental assistance.",

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doi = "10.1038/nchembio.1043",

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AU - Bianco, Ambra

AU - Townsley, Fiona M.

AU - Greiss, Sebastian

AU - Lang, Kathrin

AU - Chin, Jason W.

N1 - Funding Information: This work was funded by the UK Medical Research Council (MRC) (grants U105181009 and UD99999908) and the Louis-Jeantet Foundation Young Investigator Career Award (both to J.W.C.). We are grateful to S. Bullock (MRC Laboratory of Molecular Biology) for providing initial plasmids and for extensive help and advice with this project; to S. Peak-Chew and M. Skehel in the MRC Laboratory of Molecular Biology Mass Spectrometry Facility for MS; S. Brunner for assistance with statistical analysis; and J. Schmitz and R. Pisa for experimental assistance.

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AB - Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.

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JO - Nature Chemical Biology

JF - Nature Chemical Biology

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Expanding the genetic code of Drosophila melanogaster

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