Exopolysaccharide and kestose production by Lactobacillus sanfranciscensis LTH2590

Maher Korakli, Melanie Pavlovic, Michael G. Gänzle, Rudi F. Vogel

Research output: Contribution to journalArticlepeer-review

114 Scopus citations

Abstract

The effect was investigated of sucrose concentration on sucrose metabolism and on the formation of exopolysaccharide (EPS) by Lactobacillus sanfranciscensis LTH2590 in pH-controlled fermentations with sucrose concentrations ranging from 20 to 160 g liter-1. The EPS production increased and the relative sucrose hydrolysis activity decreased by increasing the sucrose concentration in the medium. The carbon recovery decreased from 95% at a sucrose concentration of 30 g liter-1 to 58% at a sucrose concentration of 160 g liter-1 because of the production of an unknown metabolite by L. sanfranciscensis. This metabolite was characterized as a fructo-oligosaccharide. The oligosaccharide produced by L. sanfranciscensis was purified and characterized as a trisaccharide with a glucose/fructose ratio of 1:2. The comparison of the retention time of this oligosaccharide and that of pure oligosaccharide standards using two different chromatography methods revealed that the oligosaccharide produced by L. sanfranciscensis LTH2590 is 1-kestose. Kestose production increased concomitantly with the initial sucrose concentration in the medium.

Original languageEnglish
Pages (from-to)2073-2079
Number of pages7
JournalApplied and Environmental Microbiology
Volume69
Issue number4
DOIs
StatePublished - 1 Apr 2003

Fingerprint

Dive into the research topics of 'Exopolysaccharide and kestose production by Lactobacillus sanfranciscensis LTH2590'. Together they form a unique fingerprint.

Cite this