TY - JOUR
T1 - Evaluation of the suitability of recombinant yeast-based estrogenicity assays as a pre-screening tool in environmental samples
AU - Brix, Rikke
AU - Noguerol, Tania Noelia
AU - Piña, Benjamin
AU - Balaam, Jan
AU - Nilsen, Anja Julie
AU - Tollefsen, Knut Erik
AU - Levy, Walkiria
AU - Schramm, Karl Werner
AU - Barceló, Damià
N1 - Funding Information:
This work has been supported by the EU Projects: Screening methods for water data information, in support of implementation of the Water Framework Directive (SWIFT WFD, Contract SSPI-CT-2003-502492 ), MODELKEY [ GOCE 511237 ] and by the Spanish Ministry of Science and Innovation through the projects CEMAGUA ( CGL2007-64551/HID ), SCARCE (Consolider-Ingenio 2010 CSD2009-00065 ) and ( BIO2005-00840 ). Jan Balaam would like to thank the Department for Environment, Food and Rural Affairs for financial support of this work. Knut-Erik Tollefsen would like to acknowledge funding from the Norwegian Research Council , project no. 162867 . The authors would further like to acknowledge the comments from an anonymous reviewer, which improved the quality of the manuscript. The paper reflects the authors' views only. The EU is not liable for any use that may be made of the information contained in it.
PY - 2010/5
Y1 - 2010/5
N2 - This paper presents a study evaluating the suitability of recombinant yeast-based estrogenicity assays as a pre-screening tool for monitoring of the chemical status of water bodies in support of the Water Framework Directive (WFD). Three different recombinant yeast-based assays were evaluated; the Yeast Estrogen Screen (YES), the Recombinant Yeast Assay (RYA) and the Rikilt Estrogen bioAssay (REA), of which the YES assay was employed by two different laboratories. No significant difference between the performance of neither the different laboratories, nor the different yeast-assays was observed.Six batches of eleven samples each were analysed one week apart by the four participating laboratories and the robustness, repeatability and reproducibility of the participating yeast-based assays were evaluated.The setup included a correlation between bioassay results and results from chemical target analysis, which gave valuable information in the evaluation of the assays' performance. A good agreement was found between chemical and bioassay results, showing that the yeast-based assays can give valuable information in WFD work. However, the low sensitivity of the assays towards alkylphenols needs to be significantly improved if they are to be used for monitoring of these compounds. The study further led to suggestions on ways to improve traceability and quality assurance of the yeast-based assays.
AB - This paper presents a study evaluating the suitability of recombinant yeast-based estrogenicity assays as a pre-screening tool for monitoring of the chemical status of water bodies in support of the Water Framework Directive (WFD). Three different recombinant yeast-based assays were evaluated; the Yeast Estrogen Screen (YES), the Recombinant Yeast Assay (RYA) and the Rikilt Estrogen bioAssay (REA), of which the YES assay was employed by two different laboratories. No significant difference between the performance of neither the different laboratories, nor the different yeast-assays was observed.Six batches of eleven samples each were analysed one week apart by the four participating laboratories and the robustness, repeatability and reproducibility of the participating yeast-based assays were evaluated.The setup included a correlation between bioassay results and results from chemical target analysis, which gave valuable information in the evaluation of the assays' performance. A good agreement was found between chemical and bioassay results, showing that the yeast-based assays can give valuable information in WFD work. However, the low sensitivity of the assays towards alkylphenols needs to be significantly improved if they are to be used for monitoring of these compounds. The study further led to suggestions on ways to improve traceability and quality assurance of the yeast-based assays.
KW - Bioassay
KW - Endocrine disruption
KW - Screening methods
KW - Wastewater
UR - http://www.scopus.com/inward/record.url?scp=77950951063&partnerID=8YFLogxK
U2 - 10.1016/j.envint.2010.02.004
DO - 10.1016/j.envint.2010.02.004
M3 - Article
AN - SCOPUS:77950951063
SN - 0160-4120
VL - 36
SP - 361
EP - 367
JO - Environment International
JF - Environment International
IS - 4
ER -