TY - JOUR
T1 - Evaluation of the Biodistribution of Mesenchymal Stem Cells in a Pre-clinical Renal Tuberculosis Model by Non-linear Magnetic Response Measurements
AU - Yudintceva, Natalia
AU - Mikhailova, Natalia
AU - Bobkov, Danila
AU - Yakovleva, Liudmila
AU - Nikolaev, Boris
AU - Krasavina, Darya
AU - Muraviov, Alexandr
AU - Vinogradova, Tatiana
AU - Yablonskiy, Petr
AU - Samusenko, Igor
AU - Ryzhov, Vyacheslav
AU - Deriglazov, Vladimir
AU - Marchenko, Yaroslav
AU - Multhoff, Gabriele
AU - Klapproth, Alexander P.
AU - Li, Wei Bo
AU - Nayak, Barsa
AU - Sonawane, Avinash
AU - Shevtsov, Maxim
N1 - Publisher Copyright:
© Copyright © 2021 Yudintceva, Mikhailova, Bobkov, Yakovleva, Nikolaev, Krasavina, Muraviov, Vinogradova, Yablonskiy, Samusenko, Ryzhov, Deriglazov, Marchenko, Multhoff, Klapproth, Li, Nayak, Sonawane and Shevtsov.
PY - 2021/4/28
Y1 - 2021/4/28
N2 - Bone-marrow derived mesenchymal stem cells (MSCs) exert anti-tuberculosis effects due to their potential to repair damaged tissues and modulate inflammatory immune responses. MSCs were reported to be recruited to the Mycobacterium tuberculosis (Mtb) affected sites in the organism. However, due to limitations of presently applied in vivo imaging techniques the trafficking and biodistribution of MSCs in Mtb-infected organisms is not possible. In the current study MSCs were labeled with superparamagnetic iron oxide nanoparticles (SPIONs) as a negative MR contrast agent for imaging the biodistribution of MSCs in vivo. Trafficking of SPIONs-labeled MSCs was analyzed in a preclinical model of renal tuberculosis in male Chinchilla rabbits (n = 18) following intravenous administration on the days 0, 2, 3, and 7 employing a highly sensitive method of non-linear longitudinal magnetic response (NLR-M2) measurements. Within 48 h after injection, nanoparticle-labeled MSCs accumulated predominantly in lung, spleen, liver tissues, and paratracheal lymph nodes with subsequent decrease over the observation period of 7 days. The recruitment of MSCs to Mtb-affected organs was further proven by immunohistological analysis. NLR-M2 allowed the detection of SPIONs-labeled cells at low concentrations in different organs and tissues giving insights of in vivo mesenchymal stem cells trafficking in organism after TB infection.
AB - Bone-marrow derived mesenchymal stem cells (MSCs) exert anti-tuberculosis effects due to their potential to repair damaged tissues and modulate inflammatory immune responses. MSCs were reported to be recruited to the Mycobacterium tuberculosis (Mtb) affected sites in the organism. However, due to limitations of presently applied in vivo imaging techniques the trafficking and biodistribution of MSCs in Mtb-infected organisms is not possible. In the current study MSCs were labeled with superparamagnetic iron oxide nanoparticles (SPIONs) as a negative MR contrast agent for imaging the biodistribution of MSCs in vivo. Trafficking of SPIONs-labeled MSCs was analyzed in a preclinical model of renal tuberculosis in male Chinchilla rabbits (n = 18) following intravenous administration on the days 0, 2, 3, and 7 employing a highly sensitive method of non-linear longitudinal magnetic response (NLR-M2) measurements. Within 48 h after injection, nanoparticle-labeled MSCs accumulated predominantly in lung, spleen, liver tissues, and paratracheal lymph nodes with subsequent decrease over the observation period of 7 days. The recruitment of MSCs to Mtb-affected organs was further proven by immunohistological analysis. NLR-M2 allowed the detection of SPIONs-labeled cells at low concentrations in different organs and tissues giving insights of in vivo mesenchymal stem cells trafficking in organism after TB infection.
KW - SPIONs
KW - biodistribution
KW - mesenchymal stem cells
KW - non-linear magnetic response
KW - renal tuberculosis
KW - superparamagnetic iron oxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85105915540&partnerID=8YFLogxK
U2 - 10.3389/fphy.2021.625622
DO - 10.3389/fphy.2021.625622
M3 - Article
AN - SCOPUS:85105915540
SN - 2296-424X
VL - 9
JO - Frontiers in Physics
JF - Frontiers in Physics
M1 - 625622
ER -
