Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa

Julia Giebler; Lukas L. Wick; Michael Schloter; Hauke Harms; Antonis Chatzinotas

doi:10.1128/AEM.04028-12

Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa

Julia Giebler
, Lukas L. Wick
, Michael Schloter
, Hauke Harms
, Antonis Chatzinotas

Helmholtz-Zentrum für Umweltforschung (UFZ)
Helmholtz Zentrum München German Research Center for Environmental Health

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.

Original language	English
Pages (from-to)	3129-3132
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	9
DOIs	https://doi.org/10.1128/AEM.04028-12
State	Published - May 2013
Externally published	Yes

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title = "Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa",

abstract = "Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.",

author = "Julia Giebler and Wick, \{Lukas L.\} and Michael Schloter and Hauke Harms and Antonis Chatzinotas",

year = "2013",

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AU - Giebler, Julia

AU - Wick, Lukas L.

AU - Schloter, Michael

AU - Harms, Hauke

AU - Chatzinotas, Antonis

PY - 2013/5

Y1 - 2013/5

N2 - Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.

AB - Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.

UR - https://www.scopus.com/pages/publications/84876340904

U2 - 10.1128/AEM.04028-12

DO - 10.1128/AEM.04028-12

M3 - Article

C2 - 23455350

AN - SCOPUS:84876340904

SN - 0099-2240

VL - 79

SP - 3129

EP - 3132

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 9

ER -

Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa

Abstract

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