Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa

Julia Giebler, Lukas L. Wick, Michael Schloter, Hauke Harms, Antonis Chatzinotas

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.

Original languageEnglish
Pages (from-to)3129-3132
Number of pages4
JournalApplied and Environmental Microbiology
Volume79
Issue number9
DOIs
StatePublished - May 2013
Externally publishedYes

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