Estimation of the optimum ratio of standardized ileal digestible isoleucine to lysine for eight- to twenty-five-kilogram pigs in diets containing spray-dried blood cells or corn gluten feed as a protein source

Two growth assays and 1 N balance trial were conducted to determine the standardized ileal digestible (SID) Ile: Lys ratio in 8- to 25-kg pigs using spray-dried blood cells or corn gluten feed as a protein source. In Exp. 1, 48 individually penned pigs (initial BW = 7.7 kg) were used in a 6-point SID Ile titration study (analyzed SID Ile of 0.36, 0.43, 0.50, 0.57, 0.64, and 0.72%) by addition of graded levels of l-Ile. The basal diet contained 1.00% SID Lys, 18.4% CP, and 13.6 MJ of ME/kg. Diets were based on wheat, barley, corn, and 7.5% spray-dried blood cells as a protein source. Dietary SID Leu and Val levels were 1.61 and 1.02%, respectively. For the 35-d period, ADG, ADFI, and G:F increased linearly (P < 0.01) and quadratically (P < 0.04) with increasing SID Ile: Lys. Estimates of optimal SID Ile: Lys ratios were 59% for ADG and ADFI. In Exp. 2, 24 N balances were conducted using the Exp. 1 diets (12 pigs; individually penned; average BW = 11.5 kg). Pigs were fed 3 times daily with an amount equal to 1.0 MJ of ME/kg of BW^0.75. Preparation and collection periods (7 d each) were repeated after rearranging the animals to treatments. Increasing the dietary SID Ile: Lys ratio increased N retention linearly (P < 0.01), and N utilization linearly (P < 0.01) and quadratically (P < 0.01). An optimal SID Ile: Lys ratio of 54% was estimated for N retention. In Exp. 3, 48 individually penned pigs (initial BW = 8.0 kg) were fed grain-based diets in a 6-point SID Ile titration (analyzed SID Ile of 0.35, 0.41, 0.49, 0.56, 0.62, and 0.69%). Dietary SID Ile was increased by graded addition of l-Ile. The basal diet contained 0.97% SID Lys, 16.8% CP, and 13.6 MJ of ME/kg. In contrast to Exp. 1 and 2, spray-dried blood cells were excluded and corn gluten feed was used as a protein source. Dietary SID Leu and Val were set to 1.05 and 0.66%. For the 42-d period, ADG, ADFI, and G:F increased linearly (P < 0.01) and quadratically (P < 0.01) with increasing SID Ile: Lys. Estimated optimal SID Ile: Lys ratios were 54, 54, and 49 for ADG, ADFI, and G:F, respectively. These experiments suggest that the optimal SID Ile: Lys ratio depends on diet composition. In Exp. 1, AA imbalances because of increased Leu contents may have led to increased Ile nutritional needs. For ADG and ADFI, an optimum SID Ile: Lys ratio of 54% was estimated for 8- to 25-kg pigs in diets without Leu excess.