TY - JOUR
T1 - Esterification of bio-based succinic acid in biphasic systems
T2 - Comparison of chemical and biological catalysts
AU - Delhomme, Clara
AU - Goh, Serena L.M.
AU - Kühn, Fritz E.
AU - Weuster-Botz, Dirk
N1 - Funding Information:
This work was generously supported by the International Graduate School of Science and Engineering (IGSSE) at the Technische Universität München, particularly by an IGSSE Ph.D. grant for Dr. Clara Delhomme. Dr. Serena L.M. Goh is grateful to the Bavarian Research Foundation for a Ph.D. grant. The assistance of Elona Hasanbelli was greatly appreciated.
PY - 2012/8
Y1 - 2012/8
N2 - Different chemical and biological catalysts were screened for the biphasic esterification of aqueous solutions of succinic acid with 1-octanol. Among them, DBSA, Nafion NR-50 and Novozym 435 were found to be the most attractive catalysts. The pH, the temperature and the catalyst concentration had high impacts on the reaction rates. The optimization of the reaction conditions with a single-variable approach for the chemical catalysts and a Response Surface Methodology for the enzyme allowed an increase of the rates by a factor 1.5 for DBSA, 2.3 for Nafion NR-50 and 1.3 for Novozym 435. Real fermentation broths produced from recombinant Escherichia coli could be successfully esterified with conversions up to 93% for DBSA and 70% for Nafion NR-50 and Novozym 435 as catalysts. Finally, DBSA was selected as the cheapest and most active option. Besides, the esters were isolated with a purity of 83% from fermentation broth solutions. DBSA also catalyzed the esterification of succinic acid from fermentation broth with many alcohols creating a broad spectrum of interesting esters. The esters might then be used as end-product, hydrolyzed back to pure succinic acid or hydrogenated.
AB - Different chemical and biological catalysts were screened for the biphasic esterification of aqueous solutions of succinic acid with 1-octanol. Among them, DBSA, Nafion NR-50 and Novozym 435 were found to be the most attractive catalysts. The pH, the temperature and the catalyst concentration had high impacts on the reaction rates. The optimization of the reaction conditions with a single-variable approach for the chemical catalysts and a Response Surface Methodology for the enzyme allowed an increase of the rates by a factor 1.5 for DBSA, 2.3 for Nafion NR-50 and 1.3 for Novozym 435. Real fermentation broths produced from recombinant Escherichia coli could be successfully esterified with conversions up to 93% for DBSA and 70% for Nafion NR-50 and Novozym 435 as catalysts. Finally, DBSA was selected as the cheapest and most active option. Besides, the esters were isolated with a purity of 83% from fermentation broth solutions. DBSA also catalyzed the esterification of succinic acid from fermentation broth with many alcohols creating a broad spectrum of interesting esters. The esters might then be used as end-product, hydrolyzed back to pure succinic acid or hydrogenated.
KW - Biphasic esterification
KW - DBSA
KW - Nafion NR-50
KW - Novozym 435
KW - Reactive extraction
KW - Succinic acid
UR - http://www.scopus.com/inward/record.url?scp=84861002279&partnerID=8YFLogxK
U2 - 10.1016/j.molcatb.2012.03.010
DO - 10.1016/j.molcatb.2012.03.010
M3 - Article
AN - SCOPUS:84861002279
SN - 1381-1177
VL - 80
SP - 39
EP - 47
JO - Journal of Molecular Catalysis B: Enzymatic
JF - Journal of Molecular Catalysis B: Enzymatic
ER -