EPR-based approach for the localization of paramagnetic metal Ions in biomolecules

Dinar Abdullin; Nicole Florin; Gregor Hagelueken; Olav Schiemann

doi:10.1002/anie.201410396

EPR-based approach for the localization of paramagnetic metal Ions in biomolecules

Dinar Abdullin, Nicole Florin, Gregor Hagelueken, Olav Schiemann

University of Bonn

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three-dimensional fold of such biomolecules is therefore an important goal in understanding structure-function relationships. A trilateration approach for the localization of metal ions by means of long-range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu²⁺ center of azurin, and factors affecting the precision of the method are discussed.

Original language	English
Pages (from-to)	1827-1831
Number of pages	5
Journal	Angewandte Chemie International Edition in English
Volume	54
Issue number	6
DOIs	https://doi.org/10.1002/anie.201410396
State	Published - 9 Feb 2015
Externally published	Yes

Keywords

Binding sites
Copper
Epr spectroscopy
Spin labels ,triangulation

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10.1002/anie.201410396

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abstract = "Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three-dimensional fold of such biomolecules is therefore an important goal in understanding structure-function relationships. A trilateration approach for the localization of metal ions by means of long-range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu2+ center of azurin, and factors affecting the precision of the method are discussed.",

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AU - Hagelueken, Gregor

AU - Schiemann, Olav

PY - 2015/2/9

Y1 - 2015/2/9

N2 - Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three-dimensional fold of such biomolecules is therefore an important goal in understanding structure-function relationships. A trilateration approach for the localization of metal ions by means of long-range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu2+ center of azurin, and factors affecting the precision of the method are discussed.

AB - Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three-dimensional fold of such biomolecules is therefore an important goal in understanding structure-function relationships. A trilateration approach for the localization of metal ions by means of long-range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu2+ center of azurin, and factors affecting the precision of the method are discussed.

KW - Binding sites

KW - Copper

KW - Epr spectroscopy

KW - Spin labels ,triangulation

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ER -

EPR-based approach for the localization of paramagnetic metal Ions in biomolecules

Abstract

Keywords

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