TY - JOUR
T1 - Epi-laser in situ keratomileusis
T2 - Comparative evaluation of epithelial separation with 3 microkeratomes
AU - Herrmann, Wolfgang A.
AU - Hillenkamp, Jost
AU - Hufendiek, Karsten
AU - Prahs, Philipp
AU - Lohmann, Chris P.
AU - Helbig, Horst
AU - Kobuch, Karin
PY - 2008/10
Y1 - 2008/10
N2 - Purpose: To evaluate the cleavage plane, corneal cytoarchitecture, and cell vitality of separated corneal epithelial sheets created with 3 commonly used microkeratomes. Setting: Laboratories of the Regensburg University Medical Center, Regensburg, Germany. Methods: Mechanical separation of the epithelial layer in 10 porcine eyes and 2 human eyes was performed with 3 different microkeratomes (Amadeus II, Zyoptix XP, Epivision). Five of 10 porcine corneas and the 2 human corneas treated with each microkeratome were processed for histology, electron microscopy, and immunohistochemistry. In 5 of 10 porcine corneas, the corneal epithelial sheets were separated from the globe and cell vitality was assessed with the trypan blue dye vitality test. Results: A reproducible epithelial separation with a smooth surface was achieved in all eyes. The cleavage plane was located between the lamina lucida and the lamina densa. Damage to epithelial cells was mainly limited to the cut margins. Conclusions: Mechanical separation of the epithelial sheet in epithelial laser in situ keratomileusis (epi-LASIK) was safe and reproducible with all evaluated microkeratomes. Immunohistochemistry and electron microscopy showed the cleavage plane in epi-LASIK was between the basal epithelium and the basement membrane at the level of the lamina lucida.
AB - Purpose: To evaluate the cleavage plane, corneal cytoarchitecture, and cell vitality of separated corneal epithelial sheets created with 3 commonly used microkeratomes. Setting: Laboratories of the Regensburg University Medical Center, Regensburg, Germany. Methods: Mechanical separation of the epithelial layer in 10 porcine eyes and 2 human eyes was performed with 3 different microkeratomes (Amadeus II, Zyoptix XP, Epivision). Five of 10 porcine corneas and the 2 human corneas treated with each microkeratome were processed for histology, electron microscopy, and immunohistochemistry. In 5 of 10 porcine corneas, the corneal epithelial sheets were separated from the globe and cell vitality was assessed with the trypan blue dye vitality test. Results: A reproducible epithelial separation with a smooth surface was achieved in all eyes. The cleavage plane was located between the lamina lucida and the lamina densa. Damage to epithelial cells was mainly limited to the cut margins. Conclusions: Mechanical separation of the epithelial sheet in epithelial laser in situ keratomileusis (epi-LASIK) was safe and reproducible with all evaluated microkeratomes. Immunohistochemistry and electron microscopy showed the cleavage plane in epi-LASIK was between the basal epithelium and the basement membrane at the level of the lamina lucida.
UR - http://www.scopus.com/inward/record.url?scp=52049085290&partnerID=8YFLogxK
U2 - 10.1016/j.jcrs.2008.06.028
DO - 10.1016/j.jcrs.2008.06.028
M3 - Article
C2 - 18812130
AN - SCOPUS:52049085290
SN - 0886-3350
VL - 34
SP - 1761
EP - 1766
JO - Journal of Cataract and Refractive Surgery
JF - Journal of Cataract and Refractive Surgery
IS - 10
ER -