Enzyme-Linked Immunosorbent Assay for the Determination 2,4,6-Trinitrotoluene and Related Nitroaromatic Compounds

Claudia Kevchel, Ludwig Well, Reinhard Niessner

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23 Scopus citations

Abstract

A sensitive enzyme-linked Immunosorbent assay (ELISA) for the determination of the explosive 2,4,6-trinitrotoluene (TNT) and other nitroaromatic compounds was developed. As tracer, a trinitrophenyl-derivative conjugated to horseradish peroxidase was used. With this competitive assay, performed in polystyrene microtitre plates, TNT can be detected within the range of 0.02 mg/1 and 20 mg/1. Cross-reactivities at the center points (50% values) of several nitroaromatic compounds were determined. Additionally, the cross-reactivities at different concentrations of the analytes were stated to yield a more realistic estimation of the interferences. Finally, the applicability of the immunoassay to the determination of TNT in water samples was assessed.

Original languageEnglish
Pages (from-to)9-12
Number of pages4
JournalAnalytical Sciences
Volume8
Issue number1
DOIs
StatePublished - 1992

Keywords

  • Enzyme-linked immunosorbent assay
  • cross-reactivity
  • enzyme immunoassay
  • nitroaromatic compounds
  • trinitrotoluene

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