Enzyme-Linked Immunosorbent Assay for Humic Acids

Petra Ulrich; Michael G. Weller; Dietmar Knopp; Reinhard Niessner

doi:10.2116/analsci.9.795

Enzyme-Linked Immunosorbent Assay for Humic Acids

Petra Ulrich, Michael G. Weller, Dietmar Knopp, Reinhard Niessner

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

An enzyme-linked immunoassay (ELISA) for the determination of humic acid in natural-water samples was developed. For antisera production, humic acid was coupled to helix pomatia hemocyanin. A humic acid-horseradish peroxidase conjugate was synthesized as a tracer. The detection limit of this competitive assay was estimated to be 0.5 μ/1, which means 100 pg absolute. The cross-reactivities at the center point (50%-value) of several commercial humic acids and both aliphatic and aromatic acids were determined. Moreover, the structural aspects of the humic acids could be elucidated.

Original language	English
Pages (from-to)	795-797
Number of pages	3
Journal	Analytical Sciences
Volume	9
Issue number	6
DOIs	https://doi.org/10.2116/analsci.9.795
State	Published - 1993

Keywords

Humic acid
cross-reactivity
dissolved organic carbon
enzyme-linked immunoassay
polyclonal antibody
water analysis

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10.2116/analsci.9.795

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title = "Enzyme-Linked Immunosorbent Assay for Humic Acids",

abstract = "An enzyme-linked immunoassay (ELISA) for the determination of humic acid in natural-water samples was developed. For antisera production, humic acid was coupled to helix pomatia hemocyanin. A humic acid-horseradish peroxidase conjugate was synthesized as a tracer. The detection limit of this competitive assay was estimated to be 0.5 μ/1, which means 100 pg absolute. The cross-reactivities at the center point (50%-value) of several commercial humic acids and both aliphatic and aromatic acids were determined. Moreover, the structural aspects of the humic acids could be elucidated.",

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journal = "Analytical Sciences",

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T1 - Enzyme-Linked Immunosorbent Assay for Humic Acids

AU - Ulrich, Petra

AU - Weller, Michael G.

AU - Knopp, Dietmar

AU - Niessner, Reinhard

PY - 1993

Y1 - 1993

N2 - An enzyme-linked immunoassay (ELISA) for the determination of humic acid in natural-water samples was developed. For antisera production, humic acid was coupled to helix pomatia hemocyanin. A humic acid-horseradish peroxidase conjugate was synthesized as a tracer. The detection limit of this competitive assay was estimated to be 0.5 μ/1, which means 100 pg absolute. The cross-reactivities at the center point (50%-value) of several commercial humic acids and both aliphatic and aromatic acids were determined. Moreover, the structural aspects of the humic acids could be elucidated.

AB - An enzyme-linked immunoassay (ELISA) for the determination of humic acid in natural-water samples was developed. For antisera production, humic acid was coupled to helix pomatia hemocyanin. A humic acid-horseradish peroxidase conjugate was synthesized as a tracer. The detection limit of this competitive assay was estimated to be 0.5 μ/1, which means 100 pg absolute. The cross-reactivities at the center point (50%-value) of several commercial humic acids and both aliphatic and aromatic acids were determined. Moreover, the structural aspects of the humic acids could be elucidated.

KW - Humic acid

KW - cross-reactivity

KW - dissolved organic carbon

KW - enzyme-linked immunoassay

KW - polyclonal antibody

KW - water analysis

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DO - 10.2116/analsci.9.795

M3 - Article

AN - SCOPUS:84996000196

SN - 0910-6340

VL - 9

SP - 795

EP - 797

JO - Analytical Sciences

JF - Analytical Sciences

IS - 6

ER -

Enzyme-Linked Immunosorbent Assay for Humic Acids

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Keywords

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