Enzymatic esterification of free fatty acids in vegetable oils utilizing different immobilized lipases

Daniela von der Haar; Andreas Stäbler; Rolf Wichmann; Ute Schweiggert-Weisz

doi:10.1007/s10529-014-1668-1

Enzymatic esterification of free fatty acids in vegetable oils utilizing different immobilized lipases

Daniela von der Haar, Andreas Stäbler, Rolf Wichmann, Ute Schweiggert-Weisz

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Different immobilized lipases were screened for their ability to esterify free fatty acids (FFA) with monoacylglycerol (MAG) as acyl-group acceptor. A lipase from Rhizomucor miehei (Lipozyme RMIM) was the most suitable for lipase-catalyzed de-acidification—a promising alternative to conventional neutralization. A reduction of the FFA content to 0.6 % (w/w) was achieved by applying a substrate with an initial FFA-content of 6 % (w/w), the reaction at 50 °C for 22 h as well as the stepwise addition of a quadruple stoichiometric amount of MAG.

Original language	English
Pages (from-to)	169-174
Number of pages	6
Journal	Biotechnology Letters
Volume	37
Issue number	1
DOIs	https://doi.org/10.1007/s10529-014-1668-1
State	Published - 17 Jan 2015
Externally published	Yes

Keywords

Enzymatic de-acidification
Esterification
Free fatty acids
Lipase
Oil refining
Rapeseed oil refining

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10.1007/s10529-014-1668-1

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abstract = "Different immobilized lipases were screened for their ability to esterify free fatty acids (FFA) with monoacylglycerol (MAG) as acyl-group acceptor. A lipase from Rhizomucor miehei (Lipozyme RMIM) was the most suitable for lipase-catalyzed de-acidification—a promising alternative to conventional neutralization. A reduction of the FFA content to 0.6 % (w/w) was achieved by applying a substrate with an initial FFA-content of 6 % (w/w), the reaction at 50 °C for 22 h as well as the stepwise addition of a quadruple stoichiometric amount of MAG.",

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AU - Stäbler, Andreas

AU - Wichmann, Rolf

AU - Schweiggert-Weisz, Ute

PY - 2015/1/17

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AB - Different immobilized lipases were screened for their ability to esterify free fatty acids (FFA) with monoacylglycerol (MAG) as acyl-group acceptor. A lipase from Rhizomucor miehei (Lipozyme RMIM) was the most suitable for lipase-catalyzed de-acidification—a promising alternative to conventional neutralization. A reduction of the FFA content to 0.6 % (w/w) was achieved by applying a substrate with an initial FFA-content of 6 % (w/w), the reaction at 50 °C for 22 h as well as the stepwise addition of a quadruple stoichiometric amount of MAG.

KW - Enzymatic de-acidification

KW - Esterification

KW - Free fatty acids

KW - Lipase

KW - Oil refining

KW - Rapeseed oil refining

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Enzymatic esterification of free fatty acids in vegetable oils utilizing different immobilized lipases

Abstract

Keywords

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