Enhanced fed-batch fermentation of 2,3-butanediol by Paenibacillus polymyxa DSM 365

Thomas Häßler, Doris Schieder, Rupert Pfaller, Martin Faulstich, Volker Sieber

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

Fed-batch fermentations for the production of 2,3-butanediol (BDL) with Paenibacillus polymyxa DSM 365 were investigated in 2-L-fermenters. A suitable micro-aerobic set-up enabled high product selectivity of up to 98% R,R-BDL towards meso-BDL and acetoin. Up to 111gL-1 R,R-BDL within 54h could be achieved with sufficient supply of complex medium (yeast extract). To the best of the knowledge of the authors, this is the highest titer so far reported for P. polymyxa indicating its high potential as a non pathogenic BDL-producer. Fermentation in low nutritional medium (5gL-1 yeast extract) yielded up to 72gL-1 BDL+acetoin (79% R,R-BDL), yet was affected by formation of exopolysaccharides (EPS). In the range of 30-40°C EPS formation decreased with raising temperature although growth rate and BDL-production remained similar. Additionally, Tween80® was found to be a good additive to reduce viscosity caused by EPS.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalBioresource Technology
Volume124
DOIs
StatePublished - Nov 2012

Keywords

  • 2,3-Butanediol
  • Exopolysaccharides
  • Ka
  • Paenibacillus polymyxa
  • Tween80®

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