Engineering of formate dehydrogenase: Synergistic effect of mutations affecting cofactor specificity and chemical stability

Keyphrases

• Mutation Effect 100%
• Chemical Stability 100%
• Synergistic Effect 100%
• Cofactor Specificity 100%
• NADP+ 100%
• Formate Dehydrogenase 100%
• Ethyl 4-chloroacetoacetate 60%
• Nicotinamide Adenine Dinucleotide Phosphate (NADPH) 40%
• α-haloketones 40%
• Order of Magnitude 20%
• Chloro 20%
• Ethyl 20%
• Amino Acids 20%
• Specific Activity 20%
• High Catalytic Activity 20%
• Regeneration 20%
• Operational Stability 20%
• Organic Compounds 20%
• High Resistance 20%
• Cysteine 20%
• NAD(P) 20%
• Biotransformation 20%
• Oxidoreductase 20%
• 3-hydroxybutyrate 20%
• Triple mutant 20%
• Kinetic Constants 20%
• Catalytic Efficiency 20%
• Nicotinamide Adenine Dinucleotide (NAD+) 20%
• Half-saturation Constant 20%
• High Specific Activity 20%
• Mycobacterium Vaccae 20%
• NAD+ Binding 20%
• Aldo-keto Reductase 20%
• Asymmetric Reduction 20%
• Chiral Synthons 20%

Chemistry

• Chemical Stability 100%
• Nicotinamide Adenine Dinucleotide Phosphate 100%
• Cofactor 100%
• Formate Dehydrogenase 100%
• Ethyl 80%
• NADPH 40%
• Haloketone 40%
• Organic Compound 20%
• NAD(P) 20%
• 3-Hydroxybutyrate 20%
• Ketoreductase 20%
• stability 20%
• Cysteine 20%
• Amino Acid 20%

Pharmacology, Toxicology and Pharmaceutical Science

• Chemical Stability 100%
• Formate Dehydrogenase 100%
• Nicotinamide Adenine Dinucleotide Phosphate 100%
• Reduced Nicotinamide Adenine Dinucleotide Phosphate 40%
• Haloketone 40%
• Cysteine 20%
• Amino Acid 20%
• Oxidoreductase 20%
• 3 Hydroxybutyric Acid 20%
• Mycobacterium vaccae 20%