Endothelial cell-derived microparticles in allogeneic hematopoietic stem cell recipients

Verena Pihusch, Andreas Rank, Ruth Steber, Markus Pihusch, Rudolf Pihusch, Bettina Toth, Erhard Hiller, Hans Jochem Kolb

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

BACKGROUND. Alterations of microparticles derived from different cell types are described in a number of diseases associated with inflammation and hemostatic disorders. METHODS. In this prospective study, we firstly analyzed endothelial cell derived microparticles (EMP) in 19 hematopoietic stem cell recipients. Cultured human umbilical vein endothelial cells (HUVEC) stimulated with tumor necrosis factor-alpha (TNF-alpha) served as positive controls. EMP were analyzed by fluorescent activated cell sorting (FACS), detecting the particels via expression of CD62 (E-selectin) and anionic phospholipids binding to annexin V. RESULTS. EMP were not significantly influenced by conditioning regimens with non-myeloablative chemotherapy and 4 Gy total body irradiation (TBI) or by myeloablative regimens containing 12 Gy TBI. During acute graft versus host disease (aGVHD), significantly higher levels of EMP were detected than in patients without aGVHD (18.5/μl s=10.1 vs. 14.6/μl SD=11.5; P=0.004) while infectious complications did not alter EMP levels significantly. Immunosuppressive therapy with corticosteroids tendentially elevated EMP levels. HUVEC treated with TNF-alpha 1 ng/ml, 10 ng/ml and 100 ng/ml released significantly more EMP than unstimulated cultures (30.0/μl ss=13.6 vs. 126.8/μl SD=66.9, P=0.032 / vs. 683.3/μl SD=349.9; P=0.03 / vs. 489.3 s=184.4; P=0.013). CONCLUSIONS. Elevation of EMP during aGVHD might express severe endothelial cell injury within this complication after hematopoietic stem cell transplantation and might serve as a diagnostic test for early differentiation of aGVHD from other transplanted related complications.

Original languageEnglish
Pages (from-to)1405-1409
Number of pages5
JournalTransplantation
Volume81
Issue number10
DOIs
StatePublished - May 2006
Externally publishedYes

Keywords

  • Allogeneic hematopoietic stem cell transplantation
  • Endothelial cell derived microparticles
  • Graft versus host disease
  • Immunosuppression

Fingerprint

Dive into the research topics of 'Endothelial cell-derived microparticles in allogeneic hematopoietic stem cell recipients'. Together they form a unique fingerprint.

Cite this