Enantioselectivities of Uridine Diphosphate-Glucose:Monoterpenol Glucosyltransferases from Grapevine (Vitis vinifera L.)

Friedericke Bönisch; Johanna Frotscher; Sarah Stanitzek; Ernst Rühl; Oliver Bitz; Wilfried Schwab; Matthias Wüst

doi:10.1021/bk-2015-1212.ch005

Enantioselectivities of Uridine Diphosphate-Glucose:Monoterpenol Glucosyltransferases from Grapevine (Vitis vinifera L.)

Friedericke Bönisch, Johanna Frotscher, Sarah Stanitzek, Ernst Rühl, Oliver Bitz, Wilfried Schwab, Matthias Wüst

Associate Professorship of Biotechnology of Natural Products

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Aroma-active substances, like monoterpene alcohols, often occur as non-aromatic, glycosidically bound forms and accumulate during grape ripening. Although these compounds are a precious source of aroma, little is known about the enzymes catalyzing this glycosylation. Recently, heterologous expression and biochemical assays of candidate genes extracted from the V. vinifera (Vv) genome database has led to the identification of several UDP-glucose:monoterpenol β-D-glucosyltransferases (UDP-Glc GTs). Here, kinetic resolution of racemic monoterpenols is demonstrated for several of these GTs. Thus, the enantioselective "aroma-hiding" biochemistry of these enzymes is shown for the first time in grapevine.

Original language	English
Title of host publication	Importance of Chirality to Flavor Compounds
Editors	Gary Takeoka, Karl-Heinz Engel
Publisher	American Chemical Society
Pages	77-83
Number of pages	7
ISBN (Electronic)	9780841231146
DOIs	https://doi.org/10.1021/bk-2015-1212.ch005
State	Published - 2015

Publication series

Name	ACS Symposium Series
Volume	1212
ISSN (Print)	0097-6156
ISSN (Electronic)	1947-5918

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10.1021/bk-2015-1212.ch005

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Bönisch, F., Frotscher, J., Stanitzek, S., Rühl, E., Bitz, O., Schwab, W., & Wüst, M. (2015). Enantioselectivities of Uridine Diphosphate-Glucose:Monoterpenol Glucosyltransferases from Grapevine (Vitis vinifera L.). In G. Takeoka, & K.-H. Engel (Eds.), Importance of Chirality to Flavor Compounds (pp. 77-83). (ACS Symposium Series; Vol. 1212). American Chemical Society. https://doi.org/10.1021/bk-2015-1212.ch005

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abstract = "Aroma-active substances, like monoterpene alcohols, often occur as non-aromatic, glycosidically bound forms and accumulate during grape ripening. Although these compounds are a precious source of aroma, little is known about the enzymes catalyzing this glycosylation. Recently, heterologous expression and biochemical assays of candidate genes extracted from the V. vinifera (Vv) genome database has led to the identification of several UDP-glucose:monoterpenol β-D-glucosyltransferases (UDP-Glc GTs). Here, kinetic resolution of racemic monoterpenols is demonstrated for several of these GTs. Thus, the enantioselective {"}aroma-hiding{"} biochemistry of these enzymes is shown for the first time in grapevine.",

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Bönisch, F, Frotscher, J, Stanitzek, S, Rühl, E, Bitz, O, Schwab, W & Wüst, M 2015, Enantioselectivities of Uridine Diphosphate-Glucose:Monoterpenol Glucosyltransferases from Grapevine (Vitis vinifera L.). in G Takeoka & K-H Engel (eds), Importance of Chirality to Flavor Compounds. ACS Symposium Series, vol. 1212, American Chemical Society, pp. 77-83. https://doi.org/10.1021/bk-2015-1212.ch005

Enantioselectivities of Uridine Diphosphate-Glucose:Monoterpenol Glucosyltransferases from Grapevine (Vitis vinifera L.). / Bönisch, Friedericke; Frotscher, Johanna; Stanitzek, Sarah et al.
Importance of Chirality to Flavor Compounds. ed. / Gary Takeoka; Karl-Heinz Engel. American Chemical Society, 2015. p. 77-83 (ACS Symposium Series; Vol. 1212).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Bönisch, Friedericke

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AU - Stanitzek, Sarah

AU - Rühl, Ernst

AU - Bitz, Oliver

AU - Schwab, Wilfried

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Enantioselectivities of Uridine Diphosphate-Glucose:Monoterpenol Glucosyltransferases from Grapevine (Vitis vinifera L.)

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