TY - JOUR
T1 - EMMPRIN expression is involved in the development of interstitial fibrosis and tubular atrophy in human kidney allografts
AU - Kemmner, Stephan
AU - Schulte, Christian
AU - Hann von Weyhern, Claus
AU - Schmidt, Roland
AU - Baumann, Marcus
AU - Heemann, Uwe
AU - Renders, Lutz
AU - Schmaderer, Christoph
N1 - Publisher Copyright:
© 2016 John Wiley & Sons A/S.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Background: Matrix metalloproteinases (MMP) are involved in the development of interstitial fibrosis and tubular atrophy (IF/TA) in renal disease. The synthesis of MMP is activated by the extracellular matrix metalloproteinases inducer protein (EMMPRIN). To analyze the role of EMMPRIN in IF/TA, we retrospectively detected EMMPRIN expression in specimens of human renal allografts with various levels of IF/TA. Methods: Immunohistochemistry was performed to detect EMMPRIN expression. In a retrospective analysis, a total cohort of 50 specimens were divided according to BANFF-classification into four subgroups (0-3): no, mild (≤25%), moderate (26-50%), or severe (>50%) IF/TA. Among other parameters, renal function was analyzed and compared to EMMPRIN expression. Results: In 24 of 38 biopsies, we detected positive EMMPRIN staining. All nephrectomy (n = 12) samples were negative for EMMPRIN. Positive staining in the biopsy samples was detectable on the basolateral side of tubular epithelial cells. EMMPRIN staining was negatively correlated with IF/TA (p < 0.001). We found significant differences between the mean EMMPRIN expression in IF/TA groups 0 and 3 (p = 0.021) and groups 1 and 3 (p = 0.004). Furthermore, we found significant correlations between EMMPRIN staining and renal function. Conclusion: Our data suggest that EMMPRIN is involved in the pathophysiology of IF/TA.
AB - Background: Matrix metalloproteinases (MMP) are involved in the development of interstitial fibrosis and tubular atrophy (IF/TA) in renal disease. The synthesis of MMP is activated by the extracellular matrix metalloproteinases inducer protein (EMMPRIN). To analyze the role of EMMPRIN in IF/TA, we retrospectively detected EMMPRIN expression in specimens of human renal allografts with various levels of IF/TA. Methods: Immunohistochemistry was performed to detect EMMPRIN expression. In a retrospective analysis, a total cohort of 50 specimens were divided according to BANFF-classification into four subgroups (0-3): no, mild (≤25%), moderate (26-50%), or severe (>50%) IF/TA. Among other parameters, renal function was analyzed and compared to EMMPRIN expression. Results: In 24 of 38 biopsies, we detected positive EMMPRIN staining. All nephrectomy (n = 12) samples were negative for EMMPRIN. Positive staining in the biopsy samples was detectable on the basolateral side of tubular epithelial cells. EMMPRIN staining was negatively correlated with IF/TA (p < 0.001). We found significant differences between the mean EMMPRIN expression in IF/TA groups 0 and 3 (p = 0.021) and groups 1 and 3 (p = 0.004). Furthermore, we found significant correlations between EMMPRIN staining and renal function. Conclusion: Our data suggest that EMMPRIN is involved in the pathophysiology of IF/TA.
KW - CD147/basigin
KW - Chronic allograft dysfunction
KW - Extracellular matrix metalloproteinases inducer
KW - Interstitial fibrosis and tubular atrophy
KW - Kidney transplantation
KW - Matrix metalloproteinases
UR - http://www.scopus.com/inward/record.url?scp=84959514651&partnerID=8YFLogxK
U2 - 10.1111/ctr.12677
DO - 10.1111/ctr.12677
M3 - Article
C2 - 26659824
AN - SCOPUS:84959514651
SN - 0902-0063
VL - 30
SP - 218
EP - 225
JO - Clinical Transplantation
JF - Clinical Transplantation
IS - 3
ER -